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Literature summary extracted from

  • Shankar, R.A.; Anderson, P.M.
    Purification and properties of glutamine synthetase from liver of Squalus acanthias (1985), Arch. Biochem. Biophys., 239, 248-259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.2 ethylene glycol 4-8%, 50% activation Squalus acanthias

General Stability

EC Number General Stability Organism
6.3.1.2 stability is enhanced by the presence of ethylene glycol Squalus acanthias

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ca2+ inhibits in presence of Mg2+ Squalus acanthias
6.3.1.2 F-
-
 Squalus acanthias
6.3.1.2 Urea physiological concentrations of urea inhibit, at least when ATP and/or glutamate are nonsaturating. Inhibition is partially reversed by trimethylamine-N-oxide Squalus acanthias

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 mitochondrion
-
 Squalus acanthias 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ Mg2+ in excess of that required for formation of MgATP2- is required for maximal activity Squalus acanthias
6.3.1.2 Mn2+ cannot effectively replace Mg2+ Squalus acanthias

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 46000
-
 x * 46000, SDS-PAGE Squalus acanthias
6.3.1.2 200000
-
 gel filtration, in presence of Mg2+, MgATP2-, and L-Glu the enzyme has a MW of 400000, reversible dissociation from the 400000 MW form to a species of MW 200000 in the absence of MgATP2- and L-Glu Squalus acanthias
6.3.1.2 400000
-
 gel filtration, in presence of Mg2+, MgATP2-, and L-Glu, reversible dissociation to a species of MW 200000 in the absence of MgATP2- and L-Glu Squalus acanthias

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+ Squalus acanthias first step in urea synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Squalus acanthias
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 55.8
-
-
 Squalus acanthias

Storage Stability

EC Number Storage Stability Organism
6.3.1.2 -20°C, 10 mM MgSO4, 4 mM ATP, 50 mM glutamate, 100 mM NaCl, 10% ethylene glycol, 20 mM Hepes buffer, pH 7.4, less than 5% loss of activity per week Squalus acanthias

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Squalus acanthias ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+ first step in urea synthesis Squalus acanthias ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 ? x * 46000, SDS-PAGE Squalus acanthias

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.1 7.4
-
 Squalus acanthias

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.2 6.5 8 half-maximal activity at pH 6.5 and 8.0 Squalus acanthias