EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | cysteine | - |
Lupinus luteus |
EC Number | General Stability | Organism |
---|---|---|
6.3.1.2 | keto compounds, e.g. 2-oxoglutarate, oxaloacetate or pyruvate, enhance stability during purification | Lupinus luteus |
6.3.1.2 | mercaptoethanol and divalent metal ions are essential to maintain the enzyme activity | Lupinus luteus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | Ala | - |
Lupinus luteus | |
6.3.1.2 | Asp | - |
Lupinus luteus | |
6.3.1.2 | Ca2+ | strong inhibition of Mg2+-activated enzyme | Lupinus luteus | |
6.3.1.2 | Co2+ | strong inhibition of Mg2+-activated enzyme | Lupinus luteus | |
6.3.1.2 | Gly | - |
Lupinus luteus | |
6.3.1.2 | Mn2+ | strong inhibition of Mg2+-activated enzyme | Lupinus luteus | |
6.3.1.2 | additional information | combined inhibition by Gly, Ala and Ser is cumulative | Lupinus luteus | |
6.3.1.2 | phosphate | transferase assay an biosynthetic assay | Lupinus luteus | |
6.3.1.2 | Ser | - |
Lupinus luteus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.0000597 | - |
ADP | gamma-glutamyl transferase assay | Lupinus luteus | |
6.3.1.2 | 0.0125 | - |
NH4+ | biosynthetic assay | Lupinus luteus | |
6.3.1.2 | 0.22 | - |
ATP | biosynthetic assay | Lupinus luteus | |
6.3.1.2 | 3.37 | - |
hydroxylamine | gamma-glutamyl transferase assay | Lupinus luteus | |
6.3.1.2 | 8.58 | - |
Glu | biosynthetic assay | Lupinus luteus | |
6.3.1.2 | 48.6 | - |
Gln | gamma-glutamyl transferase assay | Lupinus luteus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.1.2 | cytoplasm | - |
Lupinus luteus | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | Mg2+ | optimal ratio of Mg2+ to ATP is 3:1 | Lupinus luteus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 41500 | - |
x * 41500, SDS-PAGE | Lupinus luteus |
6.3.1.2 | 347000 | - |
gel filtration | Lupinus luteus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Lupinus luteus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.2 | - |
Lupinus luteus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.1.2 | root nodule | - |
Lupinus luteus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 62 | - |
gamma-glutamylhydroxamate assay | Lupinus luteus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-Glu + NH4+ | - |
Lupinus luteus | ADP + phosphate + L-Gln | - |
? | |
6.3.1.2 | Gln + hydroxylamine + ADP | - |
Lupinus luteus | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.2 | ? | x * 41500, SDS-PAGE | Lupinus luteus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 6.5 | - |
translase assay | Lupinus luteus |
6.3.1.2 | 7.9 | - |
biosynthetic assay | Lupinus luteus |