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Literature summary extracted from

  • Chen, J.; Kennedy, I.R.
    Purification and properties of lupin nodule glutamine synthetase (1985), Phytochemistry, 24, 2167-2172.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.2 cysteine
-
Lupinus luteus

General Stability

EC Number General Stability Organism
6.3.1.2 keto compounds, e.g. 2-oxoglutarate, oxaloacetate or pyruvate, enhance stability during purification Lupinus luteus
6.3.1.2 mercaptoethanol and divalent metal ions are essential to maintain the enzyme activity Lupinus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala
-
Lupinus luteus
6.3.1.2 Asp
-
Lupinus luteus
6.3.1.2 Ca2+ strong inhibition of Mg2+-activated enzyme Lupinus luteus
6.3.1.2 Co2+ strong inhibition of Mg2+-activated enzyme Lupinus luteus
6.3.1.2 Gly
-
Lupinus luteus
6.3.1.2 Mn2+ strong inhibition of Mg2+-activated enzyme Lupinus luteus
6.3.1.2 additional information combined inhibition by Gly, Ala and Ser is cumulative Lupinus luteus
6.3.1.2 phosphate transferase assay an biosynthetic assay Lupinus luteus
6.3.1.2 Ser
-
Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.0000597
-
ADP gamma-glutamyl transferase assay Lupinus luteus
6.3.1.2 0.0125
-
NH4+ biosynthetic assay Lupinus luteus
6.3.1.2 0.22
-
ATP biosynthetic assay Lupinus luteus
6.3.1.2 3.37
-
hydroxylamine gamma-glutamyl transferase assay Lupinus luteus
6.3.1.2 8.58
-
Glu biosynthetic assay Lupinus luteus
6.3.1.2 48.6
-
Gln gamma-glutamyl transferase assay Lupinus luteus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 cytoplasm
-
Lupinus luteus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ optimal ratio of Mg2+ to ATP is 3:1 Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 41500
-
x * 41500, SDS-PAGE Lupinus luteus
6.3.1.2 347000
-
gel filtration Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 root nodule
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 62
-
gamma-glutamylhydroxamate assay Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
Lupinus luteus ADP + phosphate + L-Gln
-
?
6.3.1.2 Gln + hydroxylamine + ADP
-
Lupinus luteus gamma-Glutamylhydroxamate + NH4+ + ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 ? x * 41500, SDS-PAGE Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.5
-
translase assay Lupinus luteus
6.3.1.2 7.9
-
biosynthetic assay Lupinus luteus