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Literature summary extracted from

  • Bhandari, B.; Nicholas, D.J.D.
    Properties of glutamine synthetase of bacteroids from root nodules of Glycine max (1986), Plant Sci., 44, 89-96.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala
-
 Bradyrhizobium japonicum
6.3.1.2 ATP biosynthetic reaction Bradyrhizobium japonicum
6.3.1.2 CTP biosynthetic reaction Bradyrhizobium japonicum
6.3.1.2 Gly
-
 Bradyrhizobium japonicum
6.3.1.2 ITP biosynthetic reaction Bradyrhizobium japonicum
6.3.1.2 L-Glu competitive versus L-Gln and non-competitive versus hydroxylamine; gamma-glutamyl transferase activity Bradyrhizobium japonicum
6.3.1.2 NH4Cl glutamyl transferase reaction, competitive versus L-Gln and non-competitive versus hydroxylamine Bradyrhizobium japonicum
6.3.1.2 Ser
-
 Bradyrhizobium japonicum
6.3.1.2 UTP biosynthetic reaction Bradyrhizobium japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 2.8
-
 NH4Cl second value 8.9 mM, biphasic plot Bradyrhizobium japonicum
6.3.1.2 3.3
-
 hydroxylamine
-
 Bradyrhizobium japonicum
6.3.1.2 11.1
-
 L-Gln glutamyl transferase reaction Bradyrhizobium japonicum
6.3.1.2 12.6
-
 L-Gln biosynthetic reaction Bradyrhizobium japonicum
6.3.1.2 14.3
-
 ATP
-
 Bradyrhizobium japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 63000
-
 12 * 63000, SDS-PAGE Bradyrhizobium japonicum
6.3.1.2 760000
-
 gel filtration Bradyrhizobium japonicum

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 isoenzyme I Bradyrhizobium japonicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 bacteroid of root nodules Bradyrhizobium japonicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Bradyrhizobium japonicum ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-glutamate + NH3 NH3 in form of NH4Cl Bradyrhizobium japonicum ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bradyrhizobium japonicum gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer 12 * 63000, SDS-PAGE Bradyrhizobium japonicum