BRENDA - Enzyme Database show

Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells

Hara, S.; Miyata, A.; Yokoyama, C.; Inoue, H.; Brugger, R.; Lottspeich, F.; Ullrich, V.; Tanabe, T.; J. Biol. Chem. 269, 19897-19903 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.4
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.4
0.0073
-
prostaglandin H2
-
Bos taurus
5.3.99.4
0.009
-
prostaglandin H2
-
Bos taurus
5.3.99.4
0.0094
-
Prostaglandin H1
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.4
52000
-
x * 52000, SDS-PAGE
Bos taurus
5.3.99.4
56628
-
x * 56628, calculation from nucleotide sequence
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
Prostaglandin H2
Bos taurus
formation of prostaglandin I2, which is a powerful vasodilator and the most potent natural occuring inhibitor of platelet aggregation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.4
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.4
endothelium
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.4
2
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
prostaglandin H1
-
37472
Bos taurus
12-hydroxyheptadecadienoic acid + malondialdehyde
-
37472
Bos taurus
-
5.3.99.4
Prostaglandin H2
-
37472
Bos taurus
Prostaglandin I2
prostaglandin I2
37472
Bos taurus
-
5.3.99.4
Prostaglandin H2
-
37472
Bos taurus
Prostaglandin I2
prostaglandin I2 is readily hydrolyzed to 6-keto-prostaglandin F1alpha
37472
Bos taurus
-
5.3.99.4
Prostaglandin H2
formation of prostaglandin I2, which is a powerful vasodilator and the most potent natural occuring inhibitor of platelet aggregation
37472
Bos taurus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.4
?
x * 52000, SDS-PAGE; x * 56628, calculation from nucleotide sequence
Bos taurus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.4
2.45
-
prostaglandin H2
-
Bos taurus
5.3.99.4
26.5
-
Prostaglandin H1
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.4
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.4
0.0073
-
prostaglandin H2
-
Bos taurus
5.3.99.4
0.009
-
prostaglandin H2
-
Bos taurus
5.3.99.4
0.0094
-
Prostaglandin H1
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.4
52000
-
x * 52000, SDS-PAGE
Bos taurus
5.3.99.4
56628
-
x * 56628, calculation from nucleotide sequence
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
Prostaglandin H2
Bos taurus
formation of prostaglandin I2, which is a powerful vasodilator and the most potent natural occuring inhibitor of platelet aggregation
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.4
endothelium
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.4
2
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
prostaglandin H1
-
37472
Bos taurus
12-hydroxyheptadecadienoic acid + malondialdehyde
-
37472
Bos taurus
-
5.3.99.4
Prostaglandin H2
-
37472
Bos taurus
Prostaglandin I2
prostaglandin I2
37472
Bos taurus
-
5.3.99.4
Prostaglandin H2
-
37472
Bos taurus
Prostaglandin I2
prostaglandin I2 is readily hydrolyzed to 6-keto-prostaglandin F1alpha
37472
Bos taurus
-
5.3.99.4
Prostaglandin H2
formation of prostaglandin I2, which is a powerful vasodilator and the most potent natural occuring inhibitor of platelet aggregation
37472
Bos taurus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.4
?
x * 52000, SDS-PAGE; x * 56628, calculation from nucleotide sequence
Bos taurus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.4
2.45
-
prostaglandin H2
-
Bos taurus
5.3.99.4
26.5
-
Prostaglandin H1
-
Bos taurus