BRENDA - Enzyme Database show

Assay of prostacyclin synthase using [5,6-3H]PGH2

Tai, H.H.; Sih, C.J.; Methods Enzymol. 86, 99-105 (1982)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
microsome
-
Sus scrofa
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.4
Sus scrofa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.4
aorta
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.4
additional information
-
assay of prostacyclin synthase using [5,6-3H]prostaglandin H2
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
Prostaglandin H2
-
37466
Sus scrofa
Prostaglandin I2
prostaglandin I2
37466
Sus scrofa
-
5.3.99.4
Prostaglandin H2
-
37466
Sus scrofa
Prostaglandin I2
prostaglandin I2 is readily hydrolyzed to 6-keto-prostaglandin F1alpha
37466
Sus scrofa
-
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
microsome
-
Sus scrofa
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.4
aorta
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.4
additional information
-
assay of prostacyclin synthase using [5,6-3H]prostaglandin H2
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
Prostaglandin H2
-
37466
Sus scrofa
Prostaglandin I2
prostaglandin I2
37466
Sus scrofa
-
5.3.99.4
Prostaglandin H2
-
37466
Sus scrofa
Prostaglandin I2
prostaglandin I2 is readily hydrolyzed to 6-keto-prostaglandin F1alpha
37466
Sus scrofa
-