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Literature summary extracted from
- Physiological and biochemical characterization of glyoxalase I, a general marker for cell proliferation, from a soybean cell suspension (1993), Planta, 189, 561-566.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.5 | 0.46 | - |
methylglyoxal/glutathione hemithioacetal | - |
Glycine max |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 26000 | - |
1 * 26000 + 1 * 29000, SDS-PAGE | Glycine max |
| 4.4.1.5 | 29000 | - |
1 * 26000 + 1 * 29000, SDS-PAGE | Glycine max |
| 4.4.1.5 | 60000 | - |
gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.5 | additional information | Glycine max | the enzyme is associated with cell proliferation, the activity is modulated during the proliferation cycle with a maximal activity between day 2 and day 4 of culture growth | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.5 | Glycine max | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.5 | - |
Glycine max |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | additional information | - |
- |
Glycine max |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.5 | glutathione + methylglyoxal | the substrat is the hemithioacetal of methylglyoxal and glutathione | Glycine max | S-lactoylglutathione | - |
? | |
| 4.4.1.5 | glutathione-methylglyoxal hemithioacetal | - |
Glycine max | (R)-S-lactoylglutathione | - |
? | |
| 4.4.1.5 | additional information | the enzyme is associated with cell proliferation, the activity is modulated during the proliferation cycle with a maximal activity between day 2 and day 4 of culture growth | Glycine max | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.5 | dimer | 1 * 26000 + 1 * 29000, SDS-PAGE | Glycine max |
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