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Literature summary extracted from
- Purification and characterization of glyoxalase I from Hansenula mrakii (1991), J. Ferment. Bioeng., 71, 131-133.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.5 | FeSO4 | 2.0 mM, 46.5% inhibition | Cyberlindnera mrakii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.5 | 0.91 | - |
methylglyoxal | - |
Cyberlindnera mrakii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 38000 | - |
gel filtration | Cyberlindnera mrakii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.5 | Cyberlindnera mrakii | - |
IFO 0895 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.5 | - |
Cyberlindnera mrakii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 3570 | - |
- |
Cyberlindnera mrakii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.5 | glutathione + 4,5-dioxovalerate | no activity | Cyberlindnera mrakii | ? | - |
? | |
| 4.4.1.5 | glutathione + methylglyoxal | - |
Cyberlindnera mrakii | S-lactoylglutathione | - |
? | |
| 4.4.1.5 | glutathione + phenylglyoxal | no activity | Cyberlindnera mrakii | S-mandeloylglutathione | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.5 | monomer | 1 * 38000, SDS-PAGE | Cyberlindnera mrakii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 50 | - |
- |
Cyberlindnera mrakii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 4 | 40 | pH 7.0, 30 min, stable | Cyberlindnera mrakii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 6 | - |
- |
Cyberlindnera mrakii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 7.5 | 8.5 | 4°C, 24 h, stable | Cyberlindnera mrakii |
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Release 2023.1 (January 2023)
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