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Literature summary extracted from

  • Clugston, S.L.; Barnard, J.F.J.; Kinach, R.; Miedema, D.; Ruman, R.; Daub, E.; Honek, J.F.
    Overproduction and characterization of a dimeric non-zinc glyoxalase I from Escherichia coli: evidence for optimal activation by nickel ions (1998), Biochemistry, 37, 8754-8763.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.0272
-
 glutathione-methylglyoxal hemithioacetal
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Ni2+ activates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 14919
-
 2 * 14919, electrospray mass spectrometry Escherichia coli
4.4.1.5 14990
-
 2 * 14990, SDS-PAGE Escherichia coli
4.4.1.5 30000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 additional information Escherichia coli the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids ?
-
 ?
4.4.1.5 additional information Escherichia coli MG1655/pGL10 the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Escherichia coli P0AC81 MG1655/pGL10
-
4.4.1.5 Escherichia coli MG1655/pGL10 P0AC81 MG1655/pGL10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal the substrat is the hemithioacetal of methylglyoxal and glutathione Escherichia coli S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + methylglyoxal the substrat is the hemithioacetal of methylglyoxal and glutathione Escherichia coli MG1655/pGL10 S-lactoylglutathione
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal
-
 Escherichia coli (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal
-
 Escherichia coli MG1655/pGL10 (R)-S-lactoylglutathione
-
 ?
4.4.1.5 additional information the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids Escherichia coli ?
-
 ?
4.4.1.5 additional information the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids Escherichia coli MG1655/pGL10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer 2 * 14990, SDS-PAGE Escherichia coli
4.4.1.5 dimer 2 * 14919, electrospray mass spectrometry Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 338
-
 glutathione-methylglyoxal hemithioacetal
-
 Escherichia coli