EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 0.0272 | - |
glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | Ni2+ | activates | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 14919 | - |
2 * 14919, electrospray mass spectrometry | Escherichia coli |
4.4.1.5 | 14990 | - |
2 * 14990, SDS-PAGE | Escherichia coli |
4.4.1.5 | 30000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | additional information | Escherichia coli | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | ? | - |
? | |
4.4.1.5 | additional information | Escherichia coli MG1655/pGL10 | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.5 | Escherichia coli | P0AC81 | MG1655/pGL10 | - |
4.4.1.5 | Escherichia coli MG1655/pGL10 | P0AC81 | MG1655/pGL10 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.5 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | glutathione + methylglyoxal | the substrat is the hemithioacetal of methylglyoxal and glutathione | Escherichia coli | S-lactoylglutathione | - |
? | |
4.4.1.5 | glutathione + methylglyoxal | the substrat is the hemithioacetal of methylglyoxal and glutathione | Escherichia coli MG1655/pGL10 | S-lactoylglutathione | - |
? | |
4.4.1.5 | glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli | (R)-S-lactoylglutathione | - |
? | |
4.4.1.5 | glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli MG1655/pGL10 | (R)-S-lactoylglutathione | - |
? | |
4.4.1.5 | additional information | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | Escherichia coli | ? | - |
? | |
4.4.1.5 | additional information | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | Escherichia coli MG1655/pGL10 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.5 | dimer | 2 * 14990, SDS-PAGE | Escherichia coli |
4.4.1.5 | dimer | 2 * 14919, electrospray mass spectrometry | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 338 | - |
glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli |