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Literature summary extracted from
- Cloning of a new pectate lyase gene pelC from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris (1995), Arch. Biochem. Biophys., 323, 352-360.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | pectate lyase C, expression in Pichia pastoris | Fusarium solani |
| 4.2.2.2 | pectate lyase D, expression in Pichia pastoris | Fusarium solani |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.2 | EGTA | - |
Fusarium solani |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.2 | additional information | - |
additional information | - |
Fusarium solani |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.2 | Ca2+ | required | Fusarium solani | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.2 | Fusarium solani | - |
f. sp. pisi | - |
| 4.2.2.2 | Fusarium solani | - |
pectate lyase C | - |
| 4.2.2.2 | Fusarium solani | - |
Nectria haematococca mating type VI | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.2.2.2 | side-chain modification | glycoprotein | Fusarium solani |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | pectate lyase C | Fusarium solani |
| 4.2.2.2 | recombinant enzyme | Fusarium solani |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | additional information | - |
- |
Fusarium solani |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.2 | polygalacturonic acid | endo-cleavage | Fusarium solani | unsaturated oligogalacturonate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.2 | ? | - |
Fusarium solani |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 55 | - |
- |
Fusarium solani |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 9.5 | - |
- |
Fusarium solani |
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