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Literature summary extracted from

  • Pissavin, C.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N.
    Biochemical characterization of the pectate lyase PelZ of Erwinia chrysanthemi 3937 (1998), Biochim. Biophys. Acta, 1383, 188-196.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 overproduced in Escherichia coli Dickeya chrysanthemi

General Stability

EC Number General Stability Organism
4.2.2.2 Ca2+, Mn2+ or polygalacturonate increase thermostability Dickeya chrysanthemi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 EDTA
-
 Dickeya chrysanthemi
4.2.2.2 epicatechin
-
 Dickeya chrysanthemi
4.2.2.2 iodoacetic acid
-
 Dickeya chrysanthemi
4.2.2.2 polygalacturonic acid at concentrations exceeding the Km-value Dickeya chrysanthemi
4.2.2.2 salicylic acid
-
 Dickeya chrysanthemi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.2 extracellular isoenzyme PelZ Dickeya chrysanthemi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ optimal concentration: 0.2 mM Dickeya chrysanthemi
4.2.2.2 Mn2+ optimal concentration: 0.1 mM Dickeya chrysanthemi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 40000
-
 x * 40000, isoenzyme PelZ, SDS-PAGE Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Dickeya chrysanthemi
-
 3937
-
4.2.2.2 Dickeya chrysanthemi
-
 at least 7 isoenzymes, 5 major isoenzymes, PelA, PelB, PelC, PelD, Pel E and two minor isoenzyme PelL and PelZ
-
4.2.2.2 Dickeya chrysanthemi 3937
-
 3937
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 recombinant enzyme Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectin with a low degree of methylation Dickeya chrysanthemi ?
-
 ?
4.2.2.2 pectin with a low degree of methylation Dickeya chrysanthemi 3937 ?
-
 ?
4.2.2.2 polygalacturonic acid with a low degree of methylation Dickeya chrysanthemi unsaturated oligogalacturonate
-
 ?
4.2.2.2 polygalacturonic acid with a low degree of methylation Dickeya chrysanthemi 3937 unsaturated oligogalacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 40000, isoenzyme PelZ, SDS-PAGE Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 60
-
 10 min, 50% loss of activity in absence of cations, 10% loss of activity in presence of Mn2+, Ca2+ or polygalacturonic acid Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8.5 9
-
 Dickeya chrysanthemi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 7 9 pH 7: less than 10% of maximal activity, pH 8.5-9: optimum Dickeya chrysanthemi