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Literature summary extracted from

  • Cowell, J.L.; Moser, K.; DeMoss, R.D.
    Tryptophanase from Aeromonas liquefaciens. Purification, molecular weight and some chemical, catalytic and immunochemical properties (1973), Biochim. Biophys. Acta, 315, 449-463.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.99.1 repeated slow freezing at -20°C and subsequent thawing at room temperature causes the enzyme to aggregate Aeromonas hydrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.1 Ala competitive Aeromonas hydrophila
4.1.99.1 Ala
-
 Escherichia coli
4.1.99.1 anthranilic acid competitive Aeromonas hydrophila
4.1.99.1 Phe competitive Aeromonas hydrophila
4.1.99.1 Phe
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.1 0.22
-
 L-Trp
-
 Aeromonas hydrophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.1 K+
-
 Escherichia coli
4.1.99.1 K+ K+ or NH4+ required Aeromonas hydrophila
4.1.99.1 K+ K+ or NH4+ required Paenibacillus alvei
4.1.99.1 NH4+
-
 Escherichia coli
4.1.99.1 NH4+ K+ or NH4+ required Aeromonas hydrophila
4.1.99.1 NH4+ K+ or NH4+ required Paenibacillus alvei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.1 216000
-
 aggregation to form species with MW 432000 Da, 648000 Da, and 864000 Da, sucrose density gradient centrifugation, equilibrium sedimentation Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Aeromonas hydrophila
-
-
-
4.1.99.1 Escherichia coli
-
-
-
4.1.99.1 Fusobacterium necrophorum subsp. funduliforme
-
-
-
4.1.99.1 Micrococcus aerogenes
-
-
-
4.1.99.1 Paenibacillus alvei
-
-
-
4.1.99.1 Paracolobactrum coliforme
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.1
-
 Aeromonas hydrophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.1 13.3
-
-
 Aeromonas hydrophila

Storage Stability

EC Number Storage Stability Organism
4.1.99.1 -20°C, 8-10 mg/ml in 0.1 M potassium phosphate buffer, pH 7.8, containing 0.1 mM pyridoxal phosphate and 1.0 mM dithiothreitol, stable for up to 1 year Aeromonas hydrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 L-Trp + H2O
-
 Escherichia coli indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Micrococcus aerogenes indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Aeromonas hydrophila indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Paenibacillus alvei indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Fusobacterium necrophorum subsp. funduliforme indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Paracolobactrum coliforme indole + pyruvate + NH4+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.1 pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Aeromonas hydrophila
4.1.99.1 pyridoxal 5'-phosphate dependent on Escherichia coli
4.1.99.1 pyridoxal 5'-phosphate dependent on Aeromonas hydrophila
4.1.99.1 pyridoxal 5'-phosphate dependent on Paenibacillus alvei
4.1.99.1 pyridoxal 5'-phosphate dependent on Fusobacterium necrophorum subsp. funduliforme
4.1.99.1 pyridoxal 5'-phosphate Km: 0.00209 mM Aeromonas hydrophila