EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.10 | AgNO3 | 0.2 mM, 85% inhibition | Prunus dulcis | |
4.1.2.10 | diethyl dicarbonate | 0.5 mM, 60% inhibition | Phlebodium aureum | |
4.1.2.10 | diethyl dicarbonate | 0.5 mM, 50% inhibition. 2 mM, 79% inhibition | Prunus dulcis | |
4.1.2.10 | diisopropyl fluorophosphate | 2 mM, 80% inhibition | Prunus dulcis | |
4.1.2.10 | iodoacetamide | 2 mM, 70% inhibition | Prunus dulcis | |
4.1.2.10 | additional information | the enzyme is not inhibited by sulfhydryl- or hydroxyl-modifying reagents | Phlebodium aureum | |
4.1.2.10 | phenylmethylsulfonyl fluoride | 2 mM, 50% inhibition | Prunus dulcis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.10 | 0.83 | - |
benzaldehyde | pH 5.3 | Phlebodium aureum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 20000 | - |
x * 20000, SDS-PAGE | Phlebodium aureum |
4.1.2.10 | 168000 | - |
isoenzyme A and C, gel filtration | Phlebodium aureum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.10 | Phlebodium aureum | - |
- |
- |
4.1.2.10 | Prunus dulcis | O24243 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.10 | - |
Phlebodium aureum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.10 | cyanide + benzaldehyde | - |
Phlebodium aureum | (R)-mandelonitrile | 82% yield, 99% enantiomeric excess | ? | |
4.1.2.10 | cyanide + benzaldehyde | - |
Prunus dulcis | (R)-mandelonitrile | 99% yield, 99% enantiomeric excess | ? | |
4.1.2.10 | cyanide + butanal | - |
Prunus dulcis | (2R)-2-hydroxypentanenitrile | 100% yield, 90% enantiomeric excess | ? | |
4.1.2.10 | cyanide + butanal | - |
Phlebodium aureum | (2R)-2-hydroxypentanenitrile | 42% yield, 55% enantiomeric excess | ? | |
4.1.2.10 | cyanide + pentan-2-one | - |
Prunus dulcis | (2R)-2-hydroxy-2-methylpentanenitrile | - |
? | |
4.1.2.10 | cyanide + pentan-2-one | - |
Phlebodium aureum | (2R)-2-hydroxy-2-methylpentanenitrile | 17% yield | ? | |
4.1.2.10 | cyanide + thiophene-2-carbaldehyde | - |
Prunus dulcis | (2R)-hydroxy(thiophen-2-yl)ethanenitrile | 48% yield, 87% enantiomeric excess | ? | |
4.1.2.10 | cyanide + thiophene-2-carbaldehyde | - |
Phlebodium aureum | (2R)-hydroxy(thiophen-2-yl)ethanenitrile | 87% yield, 99% enentiomeric excess | ? | |
4.1.2.10 | additional information | aliphatic carbonyls are poorly converted | Phlebodium aureum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.10 | multimer | x * 20000, SDS-PAGE | Phlebodium aureum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.10 | (R)-Mandelonitrile lyase | - |
Phlebodium aureum |
4.1.2.10 | (R)-Mandelonitrile lyase | - |
Prunus dulcis |
4.1.2.10 | PaMDL | - |
Prunus dulcis |
4.1.2.10 | PhaMDL | - |
Phlebodium aureum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 35 | 40 | - |
Phlebodium aureum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 3.5 | 7 | because of the base-catalyzed decomposition of mandelonitrile, pH dependence is only determined below pH 7.5. Activity increases from pH 3.5 to 6.2 and levels off between pH 6.2 and 7 for both isoforms | Phlebodium aureum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.10 | FAD | a flavoprotein | Prunus dulcis | |
4.1.2.10 | additional information | not a flavoprotein | Phlebodium aureum |