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Literature summary extracted from

  • Kane, S.M.; Vugrincic, C.; Finbloom, D.S.; Smith, D.W.E.
    Purification and some properties of the histidyl-tRNA synthetase from cytosol of rabbit reticulocytes (1978), Biochemistry, 17, 1509-1514.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.21 no stabilization by glycerol or His, ATP or unfractionated tRNA at concentrations similar to those in the enzyme assay Oryctolagus cuniculus
6.1.1.21 stability is reduced at protein concentrations of less than 0.015 mg/ml Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.21 p-hydroxymercuribenzoate
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.21 0.000004
-
 tRNAHisII substrate from rabbit reticuloytes Oryctolagus cuniculus
6.1.1.21 0.000006
-
 tRNAHis unfractionated substrate from rabbit liver or rabbit reticulocyte tRNAHisI Oryctolagus cuniculus
6.1.1.21 0.0005
-
 L-His
-
 Oryctolagus cuniculus
6.1.1.21 0.08
-
 His ATP Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.21 cytosol
-
 Oryctolagus cuniculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.21 KCl optimal concentration is 45 mM in absence of KCl, 5 mM in presence of 160 mM KCl Oryctolagus cuniculus
6.1.1.21 Mg2+ optimal concentration is 45 mM in absence of KCl, 5 mM in presence of 160 mM KCl Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.21 64000
-
 2 * 64000, SDS-PAGE Oryctolagus cuniculus
6.1.1.21 122000
-
 sucrose density gradient centrifugation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.21 reticulocyte
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.21 0.687
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
6.1.1.21 -85°C, stable for many months Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Oryctolagus cuniculus AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 dimer 2 * 64000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.21 additional information
-
 additional information
-
 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.21 8 8.5
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.21 6.5 9 enzyme activity does not vary much in this range Oryctolagus cuniculus