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Literature summary extracted from

  • Dixon, R.A.; Dey, P.M.; Whitehead, I.M.
    Purification and properties of chalcone isomerase from cell suspension cultures of Phaseolus vulgaris (1982), Biochim. Biophys. Acta, 715, 25-33.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.6 2',4'-Dihydroxy-4-methoxychalcone substrate inhibition at 0.025 mM and above Phaseolus vulgaris
5.5.1.6 2',4'-Dimethoxy-4-hydroxychalcone inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 3,9-dihydroxypterocarpan inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 4,2',4'-trihydroxychalcone substrate inhibition at 0.025 mM and above Phaseolus vulgaris
5.5.1.6 5,7,2',4'-Tetrahydroxy-8-(3,3-dimethylallyl)isoflavanone i.e. kievitone, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 5,7,2',4'-Tetrahydroxyisoflavanone i.e. dalbergioidin, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 5,7,2',4'-tetrahydroxyisoflavone i.e. 2'-hydroxygenistein, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 5,7,4',5'-Tetrahydroxyflavanol i.e. quercetin, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 5,7,4'-Trihydroxyflavanone
-
 Phaseolus vulgaris
5.5.1.6 5,7,4'-trihydroxyflavonol
-
 Phaseolus vulgaris
5.5.1.6 5,7,4'-trihydroxyisoflavone i.e. genistein, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 7,12-Dihydroxycoumestan i.e. coumestrol, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 7-Hydroxy-3',4'-dimethylchromanocoumaran i.e. phaseollin, inhibits isomerization of 4,2',4'-trihydroxychalcone Phaseolus vulgaris
5.5.1.6 HgCl2
-
 Phaseolus vulgaris
5.5.1.6 PCMB
-
 Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.6 0.008
-
 2',4'-Dihydroxy-4-methoxychalcone
-
 Phaseolus vulgaris
5.5.1.6 0.0089
-
 4,2',4'-trihydroxychalcone
-
 Phaseolus vulgaris
5.5.1.6 0.0133
-
 4,2',4',6'-Tetrahydroxychalcone
-
 Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.6 28000
-
 1 * 28000, SDS-PAGE Phaseolus vulgaris
5.5.1.6 30000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.6
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.6 cell suspension culture
-
 Phaseolus vulgaris
-

Storage Stability

EC Number Storage Stability Organism
5.5.1.6 -20°C, 5% glycerol, 35% loss of activity after 6 weeks Phaseolus vulgaris
5.5.1.6 4°C, 0.129 mg enzyme per ml, 95% of the original activity is retained after 6 days Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 2',4'-Dihydroxy-4-methoxychalcone
-
 Phaseolus vulgaris ?
-
 ?
5.5.1.6 4,2',4',6'-Tetrahydroxychalcone
-
 Phaseolus vulgaris 5,7,4'-Trihydroxyflavanone
-
 ?
5.5.1.6 4,2',4'-trihydroxychalcone i.e. isoliquiritigenin Phaseolus vulgaris (2S)-4',7-dihydroxyflavanone the product is liquiritigenin ?

Subunits

EC Number Subunits Comment Organism
5.5.1.6 monomer 1 * 28000, SDS-PAGE Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.6 8.2
-
 4,2',4'-trihydroxychalcone Phaseolus vulgaris