BRENDA - Enzyme Database

Metabolism of aromatic compounds by fungi. Kinetic properties and mechanism of 3-carboxy-cis,cis-muconate cyclase from Aspergillus niger

Thatcher, D.R.; Cain, R.B.; Eur. J. Biochem. 56, 193-204 (1975)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
5.5.1.5
rose-bengal-sensitized photooxidation
Aspergillus niger
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.5.1.5
2-Chloro-4-methylmuconolactone
non-competitive
Aspergillus niger
5.5.1.5
3-chloromuconate
competitive
Aspergillus niger
5.5.1.5
3-Chloromuconolactone
non-competitive
Aspergillus niger
5.5.1.5
3-methylmuconate
competitive
Aspergillus niger
5.5.1.5
adipate
-
Aspergillus niger
5.5.1.5
AsO2-
-
Aspergillus niger
5.5.1.5
AsO43-
-
Aspergillus niger
5.5.1.5
BO33-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
Br-
-
Aspergillus niger
5.5.1.5
cis-aconitate
-
Aspergillus niger
5.5.1.5
citraconate
-
Aspergillus niger
5.5.1.5
citrate
-
Aspergillus niger
5.5.1.5
Ethoxyformic anhydride
irreversible, presence of the competitive inhibitors cis-aconitate, trans-aconitate and cis,cis-muconate conferrs some protection
Aspergillus niger
5.5.1.5
F-
-
Aspergillus niger
5.5.1.5
fumarate
-
Aspergillus niger
5.5.1.5
glutaconate
-
Aspergillus niger
5.5.1.5
Glutarate
-
Aspergillus niger
5.5.1.5
H2PO2-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
I-
-
Aspergillus niger
5.5.1.5
Itaconate
-
Aspergillus niger
5.5.1.5
malate
-
Aspergillus niger
5.5.1.5
Maleate
-
Aspergillus niger
5.5.1.5
malonate
-
Aspergillus niger
5.5.1.5
mesaconate
-
Aspergillus niger
5.5.1.5
NO2-
-
Aspergillus niger
5.5.1.5
NO3-
-
Aspergillus niger
5.5.1.5
oxalate
-
Aspergillus niger
5.5.1.5
phosphate
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
pimelate
-
Aspergillus niger
5.5.1.5
S2O32-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
SO42-
-
Aspergillus niger
5.5.1.5
succinate
-
Aspergillus niger
5.5.1.5
Tartrate
-
Aspergillus niger
5.5.1.5
trans,trans-muconate
-
Aspergillus niger
5.5.1.5
trans-aconitate
-
Aspergillus niger
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5.1.5
0.057
-
3-carboxy-cis,cis-muconate
-
Aspergillus niger
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.5.1.5
Aspergillus niger
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.5.1.5
3-Carboxy-cis,cis-muconate
-
3718
Aspergillus niger
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5.1.5
5.5
6
-
Aspergillus niger
General Stability (protein specific)
EC Number
General Stability
Organism
5.5.1.5
rose-bengal-sensitized photooxidation
Aspergillus niger
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.5.1.5
2-Chloro-4-methylmuconolactone
non-competitive
Aspergillus niger
5.5.1.5
3-chloromuconate
competitive
Aspergillus niger
5.5.1.5
3-Chloromuconolactone
non-competitive
Aspergillus niger
5.5.1.5
3-methylmuconate
competitive
Aspergillus niger
5.5.1.5
adipate
-
Aspergillus niger
5.5.1.5
AsO2-
-
Aspergillus niger
5.5.1.5
AsO43-
-
Aspergillus niger
5.5.1.5
BO33-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
Br-
-
Aspergillus niger
5.5.1.5
cis-aconitate
-
Aspergillus niger
5.5.1.5
citraconate
-
Aspergillus niger
5.5.1.5
citrate
-
Aspergillus niger
5.5.1.5
Ethoxyformic anhydride
irreversible, presence of the competitive inhibitors cis-aconitate, trans-aconitate and cis,cis-muconate conferrs some protection
Aspergillus niger
5.5.1.5
F-
-
Aspergillus niger
5.5.1.5
fumarate
-
Aspergillus niger
5.5.1.5
glutaconate
-
Aspergillus niger
5.5.1.5
Glutarate
-
Aspergillus niger
5.5.1.5
H2PO2-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
I-
-
Aspergillus niger
5.5.1.5
Itaconate
-
Aspergillus niger
5.5.1.5
malate
-
Aspergillus niger
5.5.1.5
Maleate
-
Aspergillus niger
5.5.1.5
malonate
-
Aspergillus niger
5.5.1.5
mesaconate
-
Aspergillus niger
5.5.1.5
NO2-
-
Aspergillus niger
5.5.1.5
NO3-
-
Aspergillus niger
5.5.1.5
oxalate
-
Aspergillus niger
5.5.1.5
phosphate
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
pimelate
-
Aspergillus niger
5.5.1.5
S2O32-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
5.5.1.5
SO42-
-
Aspergillus niger
5.5.1.5
succinate
-
Aspergillus niger
5.5.1.5
Tartrate
-
Aspergillus niger
5.5.1.5
trans,trans-muconate
-
Aspergillus niger
5.5.1.5
trans-aconitate
-
Aspergillus niger
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5.1.5
0.057
-
3-carboxy-cis,cis-muconate
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.5.1.5
3-Carboxy-cis,cis-muconate
-
3718
Aspergillus niger
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5.1.5
5.5
6
-
Aspergillus niger