BRENDA - Enzyme Database

The isolation of collagenase and its enzymological and physico-chemical proterties

Hanada, K.; Mizutani, T.; Yamagishi, M.; Tsuji, H.; Misaki, T.; Sawada, J.; Agric. Biol. Chem. 37, 1771-1781 (1973)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.4.24.3
Ca2+ stabilizes
Pseudoalteromonas mariniglutinosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.24.3
2-mercaptoethanol
-
Pseudoalteromonas mariniglutinosa
3.4.24.3
NaCN
-
Pseudoalteromonas mariniglutinosa
3.4.24.3
SDS
surface active substance
Pseudoalteromonas mariniglutinosa
3.4.24.3
Sodiumthioglycolate
-
Pseudoalteromonas mariniglutinosa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.24.3
Ca2+
4 g-atom Calcium per mol enzyme; activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Co2+
activates at low concentration, inhibits at high concentration
Pseudoalteromonas mariniglutinosa
3.4.24.3
Mg2+
activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Mn2+
activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Sr2+
activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Zn2+
1 mol zinc per mol enzyme
Pseudoalteromonas mariniglutinosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.24.3
74000
-
gel filtration
Pseudoalteromonas mariniglutinosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.4.24.3
Collagen + H2O
Pseudoalteromonas mariniglutinosa
degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.24.3
Pseudoalteromonas mariniglutinosa
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.4.24.3
side-chain modification
glycoprotein
Pseudoalteromonas mariniglutinosa
Purification (Commentary)
EC Number
Commentary
Organism
3.4.24.3
-
Pseudoalteromonas mariniglutinosa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.24.3
culture medium
-
Pseudoalteromonas mariniglutinosa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.24.3
additional information
-
-
Pseudoalteromonas mariniglutinosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.24.3
carbobenzoxy-Gly-Pro-Leu-Gly-Pro + H2O
-
37101
Pseudoalteromonas mariniglutinosa
carbobenzoxy-Gly-Pro-Leu + Gly-Pro
-
37101
Pseudoalteromonas mariniglutinosa
?
3.4.24.3
Collagen + H2O
degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form
37101
Pseudoalteromonas mariniglutinosa
Hydrolyzed collagen
-
-
-
?
3.4.24.3
Collagen + H2O
degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro
37101
Pseudoalteromonas mariniglutinosa
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.24.3
38
-
-
Pseudoalteromonas mariniglutinosa
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.24.3
55
-
30 min, 40% loss of activity
Pseudoalteromonas mariniglutinosa
3.4.24.3
60
-
30 min, complete inactivation
Pseudoalteromonas mariniglutinosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.24.3
7
8.6
-
Pseudoalteromonas mariniglutinosa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.24.3
4.2
10.2
pH 10.2: about 85% of activity maximum; pH 4.2: about 20% of activity maximum
Pseudoalteromonas mariniglutinosa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.24.3
7.2
8.7
unstable outside the range
Pseudoalteromonas mariniglutinosa
General Stability (protein specific)
EC Number
General Stability
Organism
3.4.24.3
Ca2+ stabilizes
Pseudoalteromonas mariniglutinosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.24.3
2-mercaptoethanol
-
Pseudoalteromonas mariniglutinosa
3.4.24.3
NaCN
-
Pseudoalteromonas mariniglutinosa
3.4.24.3
SDS
surface active substance
Pseudoalteromonas mariniglutinosa
3.4.24.3
Sodiumthioglycolate
-
Pseudoalteromonas mariniglutinosa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.24.3
Ca2+
4 g-atom Calcium per mol enzyme; activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Co2+
activates at low concentration, inhibits at high concentration
Pseudoalteromonas mariniglutinosa
3.4.24.3
Mg2+
activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Mn2+
activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Sr2+
activates
Pseudoalteromonas mariniglutinosa
3.4.24.3
Zn2+
1 mol zinc per mol enzyme
Pseudoalteromonas mariniglutinosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.24.3
74000
-
gel filtration
Pseudoalteromonas mariniglutinosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.4.24.3
Collagen + H2O
Pseudoalteromonas mariniglutinosa
degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.4.24.3
side-chain modification
glycoprotein
Pseudoalteromonas mariniglutinosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.24.3
-
Pseudoalteromonas mariniglutinosa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.24.3
culture medium
-
Pseudoalteromonas mariniglutinosa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.24.3
additional information
-
-
Pseudoalteromonas mariniglutinosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.24.3
carbobenzoxy-Gly-Pro-Leu-Gly-Pro + H2O
-
37101
Pseudoalteromonas mariniglutinosa
carbobenzoxy-Gly-Pro-Leu + Gly-Pro
-
37101
Pseudoalteromonas mariniglutinosa
?
3.4.24.3
Collagen + H2O
degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form
37101
Pseudoalteromonas mariniglutinosa
Hydrolyzed collagen
-
-
-
?
3.4.24.3
Collagen + H2O
degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro
37101
Pseudoalteromonas mariniglutinosa
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.24.3
38
-
-
Pseudoalteromonas mariniglutinosa
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.24.3
55
-
30 min, 40% loss of activity
Pseudoalteromonas mariniglutinosa
3.4.24.3
60
-
30 min, complete inactivation
Pseudoalteromonas mariniglutinosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.24.3
7
8.6
-
Pseudoalteromonas mariniglutinosa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.24.3
4.2
10.2
pH 10.2: about 85% of activity maximum; pH 4.2: about 20% of activity maximum
Pseudoalteromonas mariniglutinosa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.24.3
7.2
8.7
unstable outside the range
Pseudoalteromonas mariniglutinosa