Any feedback?
Literature summary extracted from
- Clostridium histolyticum collagenase. II. Partial characterization (1972), Enzymologia, 43, 45-55.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.24.3 | Ca2+ stabilizes | Hathewaya histolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.3 | 2-mercaptoethanol | - |
Hathewaya histolytica |  |
| 3.4.24.3 | glutathione | reduced | Hathewaya histolytica | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.3 | 0.71 | - |
Carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala | - |
Hathewaya histolytica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.3 | 147200 | - |
gel filtration | Hathewaya histolytica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.3 | Collagen + H2O | Hathewaya histolytica | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.3 | Hathewaya histolytica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.3 | - |
Hathewaya histolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.3 | Azocoll + H2O | denatured collagen with bound dye, no hydrolysis | Hathewaya histolytica | Hydrolyzed azocoll | - |
? | |
| 3.4.24.3 | carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala + H2O | - |
Hathewaya histolytica | carbobenzoxy-Gly-Pro-Gly + Gly-Pro-Ala | - |
? | |
| 3.4.24.3 | Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form | Hathewaya histolytica | Hydrolyzed collagen | - |
? | |
| 3.4.24.3 | Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | Hathewaya histolytica | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.3 | additional information | - |
- |
Hathewaya histolytica |
| 3.4.24.3 | 40 | - |
3 h, Ca2+ added | Hathewaya histolytica |
| 3.4.24.3 | 50 | - |
2 h, Ca2+ added | Hathewaya histolytica |
| 3.4.24.3 | 60 | - |
5 min, complete inactivation | Hathewaya histolytica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.3 | 6.3 | 8.8 | - |
Hathewaya histolytica |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
