EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.15 | 2-mercaptoethanol | enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of 2-mercaptoethanol | Homo sapiens | |
3.4.24.15 | 2-mercaptoethanol | enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of 2-mercaptoethanol | Rattus norvegicus | |
3.4.24.15 | dithiothreitol | enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of dithiothreitol | Homo sapiens | |
3.4.24.15 | dithiothreitol | enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of dithiothreitol | Rattus norvegicus | |
3.4.24.16 | thiols | activate | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.15 | - |
Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.15 | dithiothreitol | 10 mM, reversible | Homo sapiens | |
3.4.24.15 | dithiothreitol | 10 mM, reversible | Rattus norvegicus | |
3.4.24.15 | EDTA | - |
Homo sapiens | |
3.4.24.15 | EDTA | reactivation by metal ions | Rattus norvegicus | |
3.4.24.15 | Hg2+ | - |
Homo sapiens | |
3.4.24.15 | Hg2+ | - |
Rattus norvegicus | |
3.4.24.15 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate | - |
Homo sapiens | |
3.4.24.15 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
3.4.24.15 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate | - |
Rattus norvegicus | |
3.4.24.15 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate | - |
Homo sapiens | |
3.4.24.15 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
3.4.24.15 | p-hydroxymercuribenzoate | - |
Homo sapiens | |
3.4.24.15 | p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
3.4.24.15 | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys | i.e. dynorphin-(1-13) | Homo sapiens | |
3.4.24.15 | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys | i.e. dynorphin-(1-13) | Rattus norvegicus | |
3.4.24.15 | Zn2+ | above 0.1 mM | Homo sapiens | |
3.4.24.15 | Zn2+ | above 0.1 mM | Rattus norvegicus | |
3.4.24.16 | 1,10-phenanthroline | - |
Rattus norvegicus | |
3.4.24.16 | dynorphin A(1-13) | - |
Rattus norvegicus | |
3.4.24.16 | EDTA | - |
Rattus norvegicus | |
3.4.24.16 | N-(phenylethylphosphonyl)-Gly-Pro-L-norleucine | - |
Rattus norvegicus | |
3.4.24.16 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-4-aminobenzoate | - |
Rattus norvegicus | |
3.4.24.16 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-4-aminobenzoate | - |
Rattus norvegicus | |
3.4.24.16 | N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-4-aminobenzoate | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.15 | 0.0014 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl | recombinant enzyme from E. coli | Rattus norvegicus | |
3.4.24.15 | 0.0025 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl | recombinant enzyme from E. coli | Rattus norvegicus | |
3.4.24.15 | 0.009 | - |
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl | - |
Homo sapiens | |
3.4.24.15 | 0.012 | - |
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys | - |
Rattus norvegicus | |
3.4.24.15 | 0.0133 | - |
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys | - |
Homo sapiens | |
3.4.24.15 | 0.0161 | - |
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys | recombinant enzyme from E. coli | Rattus norvegicus | |
3.4.24.15 | 0.023 | - |
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl | - |
Rattus norvegicus | |
3.4.24.15 | 0.0343 | - |
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl | recombinant enzyme from E. coli | Rattus norvegicus | |
3.4.24.16 | 0.0022 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl | - |
Rattus norvegicus | |
3.4.24.16 | 0.0043 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl | - |
Rattus norvegicus | |
3.4.24.16 | 0.0498 | - |
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl | abvove | Rattus norvegicus | |
3.4.24.16 | 0.05 | - |
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.16 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.15 | Zinc | contains 2 gatom of zinc per mol of enzyme, one catalytic zinc and one structural zinc | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.15 | 78300 | - |
x * 78300, calculation from nucleotide sequence | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.15 | additional information | Homo sapiens | proposed role in formation of enkephalins | ? | - |
? | |
3.4.24.15 | additional information | Rattus norvegicus | proposed role in formation of enkephalins | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.15 | Homo sapiens | - |
- |
- |
3.4.24.15 | Rattus norvegicus | - |
- |
- |
3.4.24.16 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.15 | - |
Homo sapiens |
3.4.24.16 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.15 | erythrocyte | - |
Homo sapiens | - |
3.4.24.15 | hypophysis | - |
Rattus norvegicus | - |
3.4.24.15 | testis | - |
Rattus norvegicus | - |
3.4.24.16 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.15 | additional information | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.15 | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl | - |
Rattus norvegicus | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile + Arg-Arg-Ala-Lys-dinitrophenyl | - |
? | |
3.4.24.15 | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl | - |
Rattus norvegicus | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu + Arg-Arg-Ala-Lys-dinitrophenyl | - |
? | |
3.4.24.15 | 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg | - |
Homo sapiens | 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg | - |
? | |
3.4.24.15 | 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg | - |
Rattus norvegicus | 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg | - |
? | |
3.4.24.15 | 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl | - |
Homo sapiens | 7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl | - |
? | |
3.4.24.15 | 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl | - |
Rattus norvegicus | 7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl | - |
? | |
3.4.24.15 | benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Homo sapiens | benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate | - |
? | |
3.4.24.15 | benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate | - |
? | |
3.4.24.15 | dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys | - |
Homo sapiens | dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys | - |
? | |
3.4.24.15 | dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys | - |
Rattus norvegicus | dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys | - |
? | |
3.4.24.15 | additional information | proposed role in formation of enkephalins | Homo sapiens | ? | - |
? | |
3.4.24.15 | additional information | proposed role in formation of enkephalins | Rattus norvegicus | ? | - |
? | |
3.4.24.15 | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu | i.e. neurotensin | Homo sapiens | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu | - |
? | |
3.4.24.15 | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu | i.e. neurotensin | Rattus norvegicus | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu | - |
? | |
3.4.24.15 | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O | dynorphin 1-17 | Homo sapiens | ? | - |
? | |
3.4.24.15 | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O | dynorphin 1-17 | Rattus norvegicus | ? | - |
? | |
3.4.24.16 | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl + H2O | i.e. QF37, cleavage site: Arg6-Ala7 | Rattus norvegicus | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg + Ala-Lys-dinitrophenyl | - |
? | |
3.4.24.16 | (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl + H2O | i.e. QF34, cleavage sites: Leu4-Arg5, Arg5-Arg6 | Rattus norvegicus | peptide fragments | - |
? | |
3.4.24.16 | 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.4.24.16 | dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O | - |
Rattus norvegicus | peptide fragments | - |
? | |
3.4.24.16 | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O | - |
Rattus norvegicus | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu | - |
? | |
3.4.24.16 | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O | i.e. dynorphin A(1-17), cleavage sites: Lys11-Leu12, Leu12-Lys13 | Rattus norvegicus | peptide fragments | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.15 | monomer | x * 78300, calculation from nucleotide sequence | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.16 | thimet peptidase II | - |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.15 | 8.2 | - |
above | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.15 | 8 | - |
unstable above | Rattus norvegicus |