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Literature summary extracted from
- Characterization of type I procollagen N-proteinase from fetal bovine tendon and skin. Purification of the 500-kilodalton form of the enzyme from bovine tendon (1994), J. Biol. Chem., 269, 11381-11390.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.24.14 | 50% loss of activity upon lyophilization | Bos taurus |
| 3.4.24.14 | Ca2+ stabilizes | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.14 | 1,10-phenanthroline | 100% inhibition at 1 mM | Bos taurus |  |
| 3.4.24.14 | 2-mercaptoethanol | 26% inhibition at 1 mM | Bos taurus | |
| 3.4.24.14 | ammonium persulfate | 94% inhibition at 10 micromol/ml | Bos taurus | |
| 3.4.24.14 | Co2+ | 20% inhibition at 0.01 mM | Bos taurus | |
| 3.4.24.14 | concanavalin A | 91% inhibition at 50 micromol/ml | Bos taurus | |
| 3.4.24.14 | Cu2+ | 60% inhibition at 0.01 mM | Bos taurus | |
| 3.4.24.14 | EDTA | 100% inhibition at 7.5 mM | Bos taurus | |
| 3.4.24.14 | Fetal bovine serum | 34% inhibition at concentration of 2% | Bos taurus | |
| 3.4.24.14 | heparan sulfate | 38% inhibition at 0.5 micromol/ml | Bos taurus | |
| 3.4.24.14 | heparin | 52-58% inhibition at 0.05 micromol/ml | Bos taurus | |
| 3.4.24.14 | Ni2+ | 19% inhibition at 0.01 mM | Bos taurus | |
| 3.4.24.14 | phosphate | ,46% inhibition at 2 mM | Bos taurus | |
| 3.4.24.14 | Poly-L-aspartate | 24% inhibition at 50 micromol/ml | Bos taurus | |
| 3.4.24.14 | Poly-L-glutamate | 40% inhibition at 50 micromol/ml | Bos taurus | |
| 3.4.24.14 | SDS | 79% inhibition at 50 micromol/ml | Bos taurus | |
| 3.4.24.14 | Zn2+ | 73% inhibition at 0.01 mM | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.14 | 0.000035 | - |
procollagen I | - |
Bos taurus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.14 | Ba2+ | increase of activity | Bos taurus | |
| 3.4.24.14 | Ca2+ | required for maximal activity | Bos taurus | |
| 3.4.24.14 | Mg2+ | increase of activity | Bos taurus | |
| 3.4.24.14 | Mn2+ | increase of activity | Bos taurus | |
| 3.4.24.14 | Sr2+ | increase of activity | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.14 | 500000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.14 | procollagen I + H2O | Bos taurus | - |
? | - |
? | |
| 3.4.24.14 | procollagen II + H2O | Bos taurus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.14 | Bos taurus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.14 | side-chain modification | glycoprotein | Bos taurus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.14 | from tendon | Bos taurus |
| 3.4.24.14 | partially from skin | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.14 | skin | - |
Bos taurus | - |
| 3.4.24.14 | tendon | - |
Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.24.14 | 25°C, 2 days, stable | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.14 | procollagen I + H2O | not procollagen III | Bos taurus | pCCollagen I + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
| 3.4.24.14 | procollagen I + H2O | - |
Bos taurus | ? | - |
? | |
| 3.4.24.14 | procollagen II + H2O | - |
Bos taurus | pCCollagen II + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
| 3.4.24.14 | procollagen II + H2O | - |
Bos taurus | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.14 | 40 | - |
90% of activity recovered after 60 min | Bos taurus |
| 3.4.24.14 | 55 | - |
10% of activity recovered after 60 min | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.14 | 0.08 | - |
procollagen I | - |
Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.14 | 8.3 | - |
procollagen I | Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.14 | 5.5 | - |
inactive below, procollagen I as substrate | Bos taurus |
| 3.4.24.14 | 6.7 | 9.5 | procollagen I as substrate, 45% of optimum activity at pH 6.7 and pH 9.5 | Bos taurus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.14 | 6.7 | 9.6 | - |
Bos taurus |
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