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Literature summary extracted from
- Purification and partial characterization of hatching protease of the sea urchin, Strongylocentrotus purpuratus (1977), Arch. Biochem. Biophys., 182, 696-704.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.24.12 | glycerol, 10% v/v, improves stability to either freezing or heating | Strongylocentrotus purpuratus |
| 3.4.24.12 | glycerol, 5% v/v, protects from inactivation | Strongylocentrotus purpuratus |
| 3.4.24.12 | low concentrations of albumin considerably stabilize the enzyme | Strongylocentrotus purpuratus |
| 3.4.24.12 | stable to freezing and thawing | Strongylocentrotus purpuratus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.12 | additional information | - |
additional information | - |
Strongylocentrotus purpuratus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | 28500 | - |
1 * 28500, SDS-PAGE | Strongylocentrotus purpuratus |
| 3.4.24.12 | 30000 | - |
gel filtration | Strongylocentrotus purpuratus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.12 | Strongylocentrotus purpuratus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.12 | - |
Strongylocentrotus purpuratus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.12 | hatching liquid | - |
Strongylocentrotus purpuratus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | additional information | - |
- |
Strongylocentrotus purpuratus |
| 3.4.24.12 | 1.44 | - |
- |
Strongylocentrotus purpuratus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.12 | dimethylated casein + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? |  |
| 3.4.24.12 | Dimethylcasein + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.12 | monomer | 1 * 28500, SDS-PAGE | Strongylocentrotus purpuratus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | 42 | - |
- |
Strongylocentrotus purpuratus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | 26 | 50 | 26°C: about 50% of maximal activity, 50°C: about 35% of maximal activity | Strongylocentrotus purpuratus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | 37 | - |
half-life: 0.33 h | Strongylocentrotus purpuratus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.12 | 0.72 | - |
dimethylated casein | - |
Strongylocentrotus purpuratus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | 7 | 9 | - |
Strongylocentrotus purpuratus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.12 | 6.5 | 9 | pH 6.5: about 55% of maximal activity, pH 9.0: about 45% of maximal activity | Strongylocentrotus purpuratus |
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