EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.5 | Fe3+ | - |
Rattus norvegicus | |
3.4.23.5 | Pb2+ | cathepsin D-II inhibited, cathepsin D-I hardly affected | Rattus norvegicus | |
3.4.23.5 | PCMB | - |
Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.5 | Zn2+ | slight activation of cathepsin D-II | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.23.5 | 44000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.23.5 | Rattus norvegicus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.23.5 | side-chain modification | cathepsin D-I: neutral sugar content 6%, contains mannose, glucose, galactose, fucose and glucosamine in a ratio of 8:2:1:1:5 | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.23.5 | affinity purification, 2 enzyme forms, the major form is termed cathepsin D-1, the minor form is termed cathepsin D-II | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.23.5 | spleen | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.23.5 | additional information | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.23.5 | Albumin + H2O | bovine serum albumin | Rattus norvegicus | ? | - |
? | |
3.4.23.5 | Albumin + H2O | human serum albumin | Rattus norvegicus | ? | - |
? | |
3.4.23.5 | Hemoglobin + H2O | - |
Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.23.5 | monomer | 1 * 44000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.5 | 45 | - |
cathepsin D-II | Rattus norvegicus |
3.4.23.5 | 55 | - |
cathepsin D-I | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.5 | 37 | - |
pH 7.0, 90 min, 40% loss of activity | Rattus norvegicus |
3.4.23.5 | 60 | - |
10 min, pH 3.8 or pH 7.0, loss of a large portion of activity of enzyme form cathepsin D-I and cathepsin D-II | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.5 | 3.8 | - |
10 min, 60°C, loss of a large portion of activity of enzyme form cathepsin D-I and cathepsin D-II | Rattus norvegicus |
3.4.23.5 | 7 | - |
10 min, 60°C, loss of a large portion of activity of enzyme form cathepsin D-I and cathepsin D-II | Rattus norvegicus |
3.4.23.5 | 7 | - |
37°C, 90 min, 40% loss of activity | Rattus norvegicus |