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Literature summary extracted from
- Engineering of porcine pepsin. Alteration of S1 substrate specificity of pepsin to those of fungal aspartic proteinases by site-directed mutagenesis (1997), J. Biol. Chem., 272, 18855-18861.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.1 | expression in Escherichia coli | Sus scrofa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | G78(S)S79 | differences in substrate specificity and catalytic activity | Sus scrofa |
| 3.4.23.1 | T77D | differences in substrate specificity and catalytic activity | Sus scrofa |
| 3.4.23.1 | T77D/G78(S)S79 | double mutant, differences in substrate specificity and catalytic activity | Sus scrofa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 0.04 | - |
Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu | wild-type | Sus scrofa |  |
| 3.4.23.1 | 0.06 | - |
Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu | T77D/G78(S)S79 double mutant | Sus scrofa | |
| 3.4.23.1 | 0.15 | - |
Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2 | T77D/G78(S)S79 double mutant | Sus scrofa | |
| 3.4.23.1 | 0.16 | - |
acetyl-Ala-Ala-Lys-Phe(4-NO2)-Ala-Ala-NH2 | T77D/G78(S)S79 double mutant | Sus scrofa | |
| 3.4.23.1 | 0.17 | - |
Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu | G78(S)S79 mutant | Sus scrofa | |
| 3.4.23.1 | 0.18 | - |
Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2 | T77D mutant | Sus scrofa | |
| 3.4.23.1 | 0.2 | - |
Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu | T77D mutant | Sus scrofa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.1 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.1 | expressed in Escherichia coli | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.1 | acetyl-Ala-Ala-Lys-Phe(4-NO2)-Ala-Ala-NH2 + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.4.23.1 | Hemoglobin + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.4.23.1 | Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2 + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.4.23.1 | Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu + H2O | - |
Sus scrofa | ? | - |
? | |
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