Any feedback?
Literature summary extracted from
- Purification of bleomycin hydrolase with a monoclonal antibody and its characterization (1987), Biochemistry, 26, 1574-1578.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.40 | dithiothreitol | activates | Oryctolagus cuniculus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.40 | 1-(L-trans-epoxysuccinylleucylamino)-4-guanidinobutane | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | Cu2+ | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | Fe2+ | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | leupeptin | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | N-ethylmaleimide | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | p-tosyl-L-lysine chloromethyl ketone | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | Zn2+ | - |
Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.40 | 0.79 | - |
bleomycin B2 | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | 0.79 | - |
peplomycin | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 48000 | - |
6 * 48000, SDS-PAGE | Oryctolagus cuniculus |
| 3.4.22.40 | 300000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.40 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.40 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.40 | liver | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.40 | bleomycin + H2O | bleomycin B2 | Oryctolagus cuniculus | deamido-bleomycin | - |
? | |
| 3.4.22.40 | bleomycin B2 + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.40 | peplomycin + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.40 | hexamer | 6 * 48000, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 6.8 | 7.8 | - |
Oryctolagus cuniculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
