Literature summary extracted from

Broemme, D.; Rossi, A.B.; Smeekens, S.P.; Anderson, D.C.; Payan, D.G.
Human bleomycin hydrolase: molecular cloning, sequencing, functional expression, and enzymatic characterization (1996), Biochemistry, 35, 6706-6714.

Application

EC Number	Application	Comment	Organism
3.4.22.40	medicine	-	Homo sapiens

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.40	expression in Sf9/baculovirus cells	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.22.40	1-(L-trans-epoxysuccinylleucylamino)-4-guanidinobutane	-	Homo sapiens
3.4.22.40	iodoacetate	-	Homo sapiens
3.4.22.40	NEM	-	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.22.40	52000	-	6 * 52000, SDS-PAGE	Homo sapiens
3.4.22.40	310000	-	gel filtration	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.40	Homo sapiens	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.4.22.40	815	-	-	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.40	bleomycin + H2O	bleomycin B2	Homo sapiens	deamido-bleomycin	-	?
3.4.22.40	bleomycin + H2O	bleomycin A2	Homo sapiens	deamido-bleomycin	-	?
3.4.22.40	citrulline-4-methylcoumarin 7-amide + H2O	-	Homo sapiens	citrulline + 7-amino-4-methylcoumarin	-	?
3.4.22.40	Glu-4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Glu + 7-amino-4-methylcoumarin	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.22.40	hexamer	6 * 52000, SDS-PAGE	Homo sapiens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.22.40	7.2	-	-	Homo sapiens

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.4.22.40	6	8	-	Homo sapiens

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Release 2023.1 (January 2023)