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Literature summary extracted from
- Purification, characterization, and amino acid composition of rabbit pulmonary bleomycin hydrolase (1987), Biochemistry, 26, 4213-4219.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.22.40 | stabilized by 2 mM dithiothreitol | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.40 | Cd2+ | - |
Oryctolagus cuniculus |  |
| 3.4.22.40 | Co2+ | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | Cu2+ | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | leupeptin | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | N-ethylmaleimide | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | puromycin | - |
Oryctolagus cuniculus | |
| 3.4.22.40 | Zn2+ | - |
Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.40 | 1.3 | - |
bleomycin A2 | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 50000 | - |
5 * 50000, SDS-PAGE | Oryctolagus cuniculus |
| 3.4.22.40 | 250000 | - |
gel filtration, PAGE | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.40 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.40 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.40 | lung | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.40 | bleomycin + H2O | bleomycin A2 | Oryctolagus cuniculus | deamido-bleomycin | - |
? | |
| 3.4.22.40 | bleomycin A2 + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.40 | pentamer | 5 * 50000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 37 | - |
loss of 50% activity after 10 h | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 7 | 7.5 | - |
Oryctolagus cuniculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 6 | 8 | - |
Oryctolagus cuniculus |
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