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Literature summary extracted from

  • De Lorenzo, F.; di Natale, P.; Schechter, A.N.
    Chemical and physical studies on the structure of the histidyl transfer ribonucleic acid synthetase from Salmonella typhimurium (1974), J. Biol. Chem., 249, 908-913.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.21 4°C, in dilute solutions stable for a few days Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.21 40000
-
 2 * 40000, centrifugation on guanidine HCl, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
6.1.1.21 78000
-
 high speed equilibrium centrifugation Salmonella enterica subsp. enterica serovar Typhimurium
6.1.1.21 92000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
6.1.1.21 -20°C, 50% glycerol, 2 mM 2-mercaptoethanol, stable for months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Salmonella enterica subsp. enterica serovar Typhimurium AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 dimer 2 * 40000, centrifugation on guanidine HCl, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium