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Literature summary extracted from

  • Kawada, A.; Hara, K.; Morimoto, K.; Hiruma, M.; Ishibashi, A.
    Rat epidermal cathepsin B: purification and characterization of proteolytic properties toward filaggrin and synthetic substrates (1995), Int. J. Biochem. Cell Biol., 27, 175-183.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 chymostatin
-
 Rattus norvegicus
3.4.22.1 L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Rattus norvegicus
3.4.22.1 leupeptin
-
 Rattus norvegicus
3.4.22.1 N-tosyl-lysyl chloromethylketone
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.34
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 skin epidermis Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.1 0.152
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.22.1 0.424
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
-
 Rattus norvegicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 filaggrin + H2O
-
 Rattus norvegicus hydrolyzed filaggrin
-
 ?
3.4.22.1 Proteins + H2O
-
 Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.1 50
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.1 20
-
 stable for 30 min Rattus norvegicus
3.4.22.1 30
-
 stable for 30 min Rattus norvegicus
3.4.22.1 50
-
 40% activity remaining after 30 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.1 5 7 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide as substrate, filaggrin is hydrolyzed at pH 4.0, but not at pH 7.0 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.1 5.5 6.5 stable Rattus norvegicus