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Literature summary extracted from

  • Towatari, T.; Kawabata, Y.; Katunuma, N.
    Crystallization and properties of cathepsin B from rat liver (1979), Eur. J. Biochem., 102, 279-289.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 2-mercaptoethanol activates Rattus norvegicus
3.4.22.1 cysteine activates Rattus norvegicus
3.4.22.1 dithiothreitol activates Rattus norvegicus
3.4.22.1 EDTA activates in presence of thiol compound Rattus norvegicus
3.4.22.1 thiol compounds required for full activity Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.1
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 1-tosylamide-2-phenylethyl chloromethylketone
-
 Rattus norvegicus
3.4.22.1 chymostatin
-
 Rattus norvegicus
3.4.22.1 iodoacetic acid
-
 Rattus norvegicus
3.4.22.1 L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Rattus norvegicus
3.4.22.1 leupeptin
-
 Rattus norvegicus
3.4.22.1 N-tosyl-lysyl chloromethylketone
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 22500
-
 sedimentation equilibrium centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.1 0.4
-
 benzoyl-DL-Arg-p-nitroanilide as substrate Rattus norvegicus
3.4.22.1 1.04
-
 benzoyl-DL-arginine ethyl ester Rattus norvegicus
3.4.22.1 2.12
-
 benzoyl-DL-arginamide Rattus norvegicus
3.4.22.1 2.75
-
 benzoyl-DL-Arg-beta-naphthylamide as substrate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 Aldolase + H2O
-
 Rattus norvegicus Hydrolyzed aldolase
-
 ?
3.4.22.1 benzoyl-arginine ethyl ester + H2O
-
 Rattus norvegicus benzoyl-arginine + ethanol
-
 ?
3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Rattus norvegicus benzoyl-DL-Arg + beta-naphthylamine
-
 ?
3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O
-
 Rattus norvegicus benzoyl-DL-Arg + p-nitroaniline
-
 ?
3.4.22.1 benzoyl-DL-argininamide + H2O
-
 Rattus norvegicus benzoyl-DL-arginine + NH3
-
 ?
3.4.22.1 Proteins + H2O
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.1 47
-
 stable for 15 min at pH 6 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.1 4 7
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.1 4 6.5 stable Rattus norvegicus