EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.16 | alpha-2-Macroglobulin | - |
Homo sapiens | |
3.4.22.16 | ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate | E-64-d, inhibits processing of proenzyme to synthetic analogs | Homo sapiens | |
3.4.22.16 | ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate | - |
Rattus norvegicus | |
3.4.22.16 | leupeptin | low sensitivity | Homo sapiens | |
3.4.22.16 | leupeptin | Ki: 0.005 mM; low sensitivity | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.16 | 0.097 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
3.4.22.16 | 0.15 | - |
Arg-4-methylcoumaryl-7-amide | - |
Homo sapiens | |
3.4.22.16 | 0.637 | - |
benzoyl-Arg-2-naphthylamide | - |
Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.22.1 | lysosome | - |
Homo sapiens | 5764 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.16 | 28000 | - |
gel filtration | Homo sapiens |
3.4.22.16 | 28000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.1 | Proteins + H2O | Homo sapiens | - |
? | - |
? | |
3.4.22.16 | Proteins + H2O | Homo sapiens | aminopeptidase and endopeptidase activity | ? | - |
? | |
3.4.22.16 | Proteins + H2O | Rattus norvegicus | aminopeptidase and endopeptidase activity | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.1 | Homo sapiens | - |
- |
- |
3.4.22.16 | Homo sapiens | - |
- |
- |
3.4.22.16 | Rattus norvegicus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.22.16 | side-chain modification | - |
Homo sapiens |
3.4.22.16 | side-chain modification | - |
Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.1 | - |
Homo sapiens |
3.4.22.16 | 2 methods | Homo sapiens |
3.4.22.16 | two methods | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.1 | liver | - |
Homo sapiens | - |
3.4.22.16 | liver | - |
Homo sapiens | - |
3.4.22.16 | liver | - |
Rattus norvegicus | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.22.16 | 4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA | Homo sapiens |
3.4.22.16 | 4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.1 | benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Homo sapiens | benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
3.4.22.1 | benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Homo sapiens | benzoyl-DL-Arg + p-nitroaniline | - |
? | |
3.4.22.1 | benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.1 | benzyloxycarbonyl-Arg-Arg-beta-naphthylamide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Arg-Arg + beta-naphthylamine | - |
? | |
3.4.22.1 | benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide | - |
Homo sapiens | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.1 | Proteins + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.22.16 | alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O | - |
Homo sapiens | alpha-N-benzoyl-DL-arginine + beta-naphthylamine | - |
? | |
3.4.22.16 | Arg-2-naphthylamide + H2O | - |
Homo sapiens | Arg + 2-naphthylamine | - |
ir | |
3.4.22.16 | Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | Arg + 2-naphthylamine | - |
ir | |
3.4.22.16 | Arg-4-methylcoumaryl-7-amide + H2O | conventional test substrate | Homo sapiens | Arg + 7-amino-4-methylcoumarin | - |
ir | |
3.4.22.16 | Arg-4-methylcoumaryl-7-amide + H2O | conventional test substrate | Rattus norvegicus | Arg + 7-amino-4-methylcoumarin | - |
ir | |
3.4.22.16 | benzoyl-DL-Arg-2-naphthylamide + H2O | - |
Homo sapiens | benzoyl-DL-Arg + 2-naphthylamine | - |
ir | |
3.4.22.16 | benzoyl-DL-Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | benzoyl-DL-Arg + 2-naphthylamine | - |
ir | |
3.4.22.16 | Leu-2-naphthylamide + H2O | - |
Homo sapiens | Leu + 2-naphthylamine | - |
ir | |
3.4.22.16 | Leu-2-naphthylamide + H2O | - |
Rattus norvegicus | Leu + 2-naphthylamine | - |
ir | |
3.4.22.16 | additional information | activity as endopeptidase and aminopeptidase on synthetic substrates | Homo sapiens | ? | - |
? | |
3.4.22.16 | additional information | activity as endopeptidase and aminopeptidase on synthetic substrates | Rattus norvegicus | ? | - |
? | |
3.4.22.16 | Proteins + H2O | aminopeptidase and endopeptidase activity | Homo sapiens | ? | - |
? | |
3.4.22.16 | Proteins + H2O | aminopeptidase and endopeptidase activity | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.1 | dimer | heavy and light chain result from cleavage of mature cathepsin B | Homo sapiens |
3.4.22.16 | monomer | - |
Homo sapiens |
3.4.22.16 | monomer | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.16 | 40 | - |
assay at | Homo sapiens |
3.4.22.16 | 40 | - |
assay at | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.16 | 1.82 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
3.4.22.16 | 1.82 | - |
benzoyl-Arg-2-naphthylamide | - |
Homo sapiens | |
3.4.22.16 | 2.53 | - |
Arg-4-methylcoumaryl-7-amide | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.1 | 6 | - |
- |
Homo sapiens |
3.4.22.16 | 5 | 6 | substrate azocasein | Homo sapiens |
3.4.22.16 | 5 | 6 | substrate azocasein | Rattus norvegicus |
3.4.22.16 | 6.8 | - |
- |
Rattus norvegicus |
3.4.22.16 | 6.8 | - |
synthetic substrates | Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.16 | 7 | - |
irreversible inactivation above | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.16 | 0.005 | - |
leupeptin | - |
Rattus norvegicus |