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Literature summary extracted from
- Purification and characterization of a secreted protease from Tetrahymena pyriformis (1983), Eur. J. Biochem., 132, 563-568.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | 1-tosylamide-2-phenylethyl chloromethylketone | - |
Tetrahymena pyriformis |  |
| 3.4.22.1 | Chloroquine | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | chymostatin | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | Elastatinal | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | Hg2+ | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | leupeptin | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | Mg2+ | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | N-tosyl-lysyl chloromethylketone | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | p-chloromercuribenzoate | - |
Tetrahymena pyriformis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 1.2 | - |
benzoyl-DL-Arg-beta-naphthylamide | - |
Tetrahymena pyriformis | |
| 3.4.22.1 | 1.6 | - |
benzoyl-DL-Arg-p-nitroanilide | - |
Tetrahymena pyriformis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.1 | extracellular | - |
Tetrahymena pyriformis | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 23000 | - |
gel filtration, SDS-PAGE | Tetrahymena pyriformis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | Proteins + H2O | Tetrahymena pyriformis | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | Tetrahymena pyriformis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.1 | - |
Tetrahymena pyriformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | azocasein + H2O | - |
Tetrahymena pyriformis | fragments of azocasein | - |
? | |
| 3.4.22.1 | benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Tetrahymena pyriformis | benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Tetrahymena pyriformis | benzoyl-DL-Arg + p-nitroaniline | - |
? | |
| 3.4.22.1 | denatured hemoglobin + H2O | - |
Tetrahymena pyriformis | hydrolyzed denatured hemoglobin | - |
? | |
| 3.4.22.1 | Proteins + H2O | - |
Tetrahymena pyriformis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.1 | monomer | 1 * 23000, gel filtration, SDS-PAGE | Tetrahymena pyriformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 4.5 | 5.5 | denatured hemoglobin as substrate | Tetrahymena pyriformis |
| 3.4.22.1 | 6.7 | - |
benzoyl-DL-Arg-p-nitroanilide substrate | Tetrahymena pyriformis |
| 3.4.22.1 | 8 | - |
azocasein as substrate | Tetrahymena pyriformis |
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