EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.50 | the cloned gene of prepro-API is expressed in Escherichia coli, pro-API is secreted into the periplasm and activated autocatalytically | Achromobacter lyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.50 | 6-amino-1-hexanoic acid | - |
Achromobacter lyticus | |
3.4.21.50 | acetonitrile | - |
Achromobacter lyticus | |
3.4.21.50 | Ba2+ | - |
Achromobacter lyticus | |
3.4.21.50 | benzoyl-DL-lysinal | - |
Achromobacter lyticus | |
3.4.21.50 | Benzoyl-Lys | - |
Achromobacter lyticus | |
3.4.21.50 | benzyloxycarbonyl-Leu-Leu-lysinal | - |
Achromobacter lyticus | |
3.4.21.50 | benzyloxycarbonyl-Pro-lysinal | - |
Achromobacter lyticus | |
3.4.21.50 | benzyloxycarbonyl-Val-lysinal | - |
Achromobacter lyticus | |
3.4.21.50 | Cs+ | - |
Achromobacter lyticus | |
3.4.21.50 | Cyclohexylamine | - |
Achromobacter lyticus | |
3.4.21.50 | diisopropylfluorophosphate | - |
Achromobacter lyticus | |
3.4.21.50 | ethylamine | - |
Achromobacter lyticus | |
3.4.21.50 | hydrazine | - |
Achromobacter lyticus | |
3.4.21.50 | Isobutylamine | - |
Achromobacter lyticus | |
3.4.21.50 | K+ | - |
Achromobacter lyticus | |
3.4.21.50 | Li+ | - |
Achromobacter lyticus | |
3.4.21.50 | Lys | - |
Achromobacter lyticus | |
3.4.21.50 | methylamine | - |
Achromobacter lyticus | |
3.4.21.50 | n-alkylamine | - |
Achromobacter lyticus | |
3.4.21.50 | n-amylamine | - |
Achromobacter lyticus | |
3.4.21.50 | n-butylamine | - |
Achromobacter lyticus | |
3.4.21.50 | n-Hexylamine | - |
Achromobacter lyticus | |
3.4.21.50 | n-Propylamine | - |
Achromobacter lyticus | |
3.4.21.50 | Na+ | - |
Achromobacter lyticus | |
3.4.21.50 | Nalpha-p-tosyl-L-Lys-chloromethylketone | - |
Achromobacter lyticus | |
3.4.21.50 | NH4+ | - |
Achromobacter lyticus | |
3.4.21.50 | phenylmethanesulfonyl fluoride | - |
Achromobacter lyticus | |
3.4.21.50 | Rb+ | - |
Achromobacter lyticus | |
3.4.21.50 | Zn2+ | - |
Achromobacter lyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.50 | 0.0019 | - |
Trypsinogen | - |
Achromobacter lyticus | |
3.4.21.50 | 0.07 | - |
N-benzoyl-L-Lys p-nitroanilide | - |
Achromobacter lyticus | |
3.4.21.50 | 0.08 | - |
Lys-p-nitroanilide | - |
Achromobacter lyticus | |
3.4.21.50 | 0.091 | - |
benzoyl-Lys methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 0.1 | - |
N-tosyl-L-Lys methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 0.32 | - |
benzoyl-Lys-NH2 | - |
Achromobacter lyticus | |
3.4.21.50 | 1 | - |
N-benzoyl-L-Orn methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 3.09 | - |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
3.4.21.50 | 3.11 | - |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
3.4.21.50 | 35.7 | - |
N-tosyl-L-Arg methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 41 | - |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.21.50 | 2-propanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
3.4.21.50 | Ethanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
3.4.21.50 | Methanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
3.4.21.50 | SDS | 0.1% w/v, in 40 mM Tris-HCl, pH 8, 20 min, 30°C, stable | Achromobacter lyticus |
3.4.21.50 | urea | 4 M, stable | Achromobacter lyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.50 | Achromobacter lyticus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.21.50 | proteolytic modification | the cloned gene of prepro-API is expressed in E. coli, pro-API is secreted into the periplasm and activated autocatalytically | Achromobacter lyticus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.50 | - |
Achromobacter lyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.50 | additional information | - |
- |
Achromobacter lyticus |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.21.50 | -20°C, 0.001-0.1 M Tris-HCl buffer, pH 6.0-10.9, at enzyme concentration higher than 0.1 mg/ml | Achromobacter lyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.50 | benzoyl-Lys methyl ester + H2O | - |
Achromobacter lyticus | benzoyl-Lys + methanol | - |
? | |
3.4.21.50 | benzoyl-Lys-NH2 + H2O | - |
Achromobacter lyticus | benzoyl-Lys + NH3 | - |
? | |
3.4.21.50 | benzoyl-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | benzoyl-Lys + p-nitroaniline | - |
? | |
3.4.21.50 | Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | Lys + p-nitroaniline | - |
? | |
3.4.21.50 | N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
3.4.21.50 | N-benzoyl-L-Orn methyl ester + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Orn + methanol | - |
? | |
3.4.21.50 | N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.50 | N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.50 | N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.50 | N-tosyl-L-Arg methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Arg + methanol | - |
? | |
3.4.21.50 | N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Lys + methanol | - |
? | |
3.4.21.50 | Trypsinogen + H2O | - |
Achromobacter lyticus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.50 | 40 | - |
stable | Achromobacter lyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.50 | 0.103 | - |
Trypsinogen | - |
Achromobacter lyticus | |
3.4.21.50 | 0.14 | - |
Lys-p-nitroanilide | - |
Achromobacter lyticus | |
3.4.21.50 | 0.86 | - |
benzoyl-Lys-p-nitroanilide | - |
Achromobacter lyticus | |
3.4.21.50 | 1.54 | - |
benzoyl-Lys-NH2 | - |
Achromobacter lyticus | |
3.4.21.50 | 3.02 | - |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
3.4.21.50 | 5.04 | - |
N-tosyl-L-Arg methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 10.4 | - |
N-benzoyl-L-Orn methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 32.9 | - |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
3.4.21.50 | 49 | - |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
3.4.21.50 | 225 | - |
benzoyl-Lys methyl ester | - |
Achromobacter lyticus | |
3.4.21.50 | 570 | - |
N-tosyl-L-Lys methyl ester | - |
Achromobacter lyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.50 | 7.8 | 8.2 | - |
Achromobacter lyticus |
3.4.21.50 | 8.5 | 10.7 | caseinolytic activity | Achromobacter lyticus |
3.4.21.50 | 9 | 9.5 | - |
Achromobacter lyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.50 | 4 | 10 | stable | Achromobacter lyticus |