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Literature summary extracted from
- Isolation and characterization of a lysine-specific protease from Pseudomonas aeruginosa (1986), J. Biol. Chem., 261, 11259-11265.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.50 | benzamidine | 0.1 mM, 21% inhibition | Pseudomonas aeruginosa |  |
| 3.4.21.50 | diisopropyl fluorophosphate | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | diisopropylfluorophosphate | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | Nalpha-p-tosyl-L-Lys-chloromethylketone | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | Nalpha-p-tosyl-L-lysine chloromethyl ketone | 1 mM, complete inhibition | Pseudomonas aeruginosa | |
| 3.4.21.50 | phenylmethanesulfonyl fluoride | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | PMSF | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.50 | 0.034 | - |
N-tosyl-Gly-Pro-Lys-p-nitroanilide | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | 0.034 | - |
tosyl-Gly-Pro-Lys-p-nitroanilide | pH 9.0 | Pseudomonas aeruginosa | |
| 3.4.21.50 | 0.053 | - |
N-tosyl-L-Lys methyl ester | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | 0.053 | - |
Tosyl-Lys methyl ester | pH 8.0 | Pseudomonas aeruginosa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.50 | extracellular | - |
Pseudomonas aeruginosa | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.50 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.50 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.50 | additional information | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.50 | fibrinogen + H2O | - |
Pseudomonas aeruginosa | fibrin + ? | - |
? | |
| 3.4.21.50 | Ile-Asn-Leu-Lys-Ala-leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2 | i.e. mastoparan. Cleavage sites: Ile-Asn-Leu-Lys-/-Ala-Leu-Ala-Ala-Leu-Ala-Lys-/-Lys-/-Ile-Leu-NH2 | Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.21.50 | Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2 + H2O | i.e. mastoparan, cleavage sites: Lys4-Ala5, Lys11-Lys12, Lys12-Ile13 | Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.21.50 | additional information | strict specificity: only peptide, ester, and amide bonds containing the carbonyl group of lysine are hydrolyzed | Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.21.50 | N-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | - |
Pseudomonas aeruginosa | N-tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? | |
| 3.4.21.50 | N-tosyl-L-Lys methyl ester + H2O | - |
Pseudomonas aeruginosa | N-tosyl-L-Lys + methanol | - |
? | |
| 3.4.21.50 | pyroglutamyl-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn + H2O | i.e. serum thymic factor | Pseudomonas aeruginosa | pyroglutamyl-Ala-Lys + Ser-Gln-Gly-Gly-Ser-Asn | - |
? | |
| 3.4.21.50 | serum thymic factor + H2O | cleavage of the Lys-Ser bond | Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.21.50 | tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | - |
Pseudomonas aeruginosa | tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? | |
| 3.4.21.50 | tosyl-Lys methyl ester + H2O | - |
Pseudomonas aeruginosa | tosyl-Lys + methanol | - |
? | |
| 3.4.21.50 | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr + H2O | i.e. dynorphin B | Pseudomonas aeruginosa | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys + Val-Val-Thr | - |
? | |
| 3.4.21.50 | Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr + H2O | i.e. alpha-endorphin | Pseudomonas aeruginosa | Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys + Ser-Gln-Thr-Pro-Leu-Val-Thr | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.50 | monomer | - |
Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.50 | Ps-1 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.50 | 59 | - |
N-tosyl-Gly-Pro-Lys-p-nitroanilide | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | 59 | - |
tosyl-Gly-Pro-Lys-p-nitroanilide | pH 9.0 | Pseudomonas aeruginosa | |
| 3.4.21.50 | 282 | - |
N-tosyl-L-Lys methyl ester | - |
Pseudomonas aeruginosa | |
| 3.4.21.50 | 282 | - |
Tosyl-Lys methyl ester | pH 8.0 | Pseudomonas aeruginosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.50 | 8 | - |
tosyl-Lys-methyl ester | Pseudomonas aeruginosa |
| 3.4.21.50 | 8 | - |
activity with tosyl-Lys methyl ester | Pseudomonas aeruginosa |
| 3.4.21.50 | 9 | - |
activity with tosyl-Gly-Pro-p-nitroanilide | Pseudomonas aeruginosa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.50 | 7 | 8.5 | pH 7.0: about 50% of maximal activity, pH: 8.5: about 70% of maximal activity, tosyl-Lys-methyl ester | Pseudomonas aeruginosa |
| 3.4.21.50 | 7 | 8.5 | pH 7.0: about 50% of maximal activity, pH 8.5. about 70% of maximal activity, activity with tosyl-Lys methyl ester | Pseudomonas aeruginosa |
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