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Literature summary extracted from
- Studies on procarboxypeptidase A of the spiny pacific dogfish (Squalus acanthias) (1976), Biochemistry, 9, 4680-4690.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.1 | chymotrypsin | activates | Squalus acanthias | |
| 3.4.17.1 | additional information | activation by chymotrypsin and trypsin | Squalus acanthias | |
| 3.4.17.1 | Trypsin | activates | Squalus acanthias |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.1 | 1,10-phenanthroline | - |
Squalus acanthias |  |
| 3.4.17.1 | 3-Phenylpropionic acid | Ki: 6.1 mM | Squalus acanthias | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.1 | 0.02 | - |
hippuryl-DL-beta-phenylacetic acid | - |
Squalus acanthias | |
| 3.4.17.1 | 0.21 | - |
carbobenzoxy-Gly-L-Phe | - |
Squalus acanthias | |
| 3.4.17.1 | 0.44 | - |
N-acetyl-Gly-L-phenyllactic acid | - |
Squalus acanthias | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.1 | Ca2+ | enhances activation | Squalus acanthias | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 35000 | - |
sedimentation equilibrium | Squalus acanthias |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.1 | Squalus acanthias | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.1 | - |
Squalus acanthias |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.1 | pancreas | - |
Squalus acanthias | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 8.9 | - |
- |
Squalus acanthias |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.1 | carbobenzoxy-Gly-L-Phe + H2O | - |
Squalus acanthias | carbobenzoxy-Gly + L-Phe | - |
ir | |
| 3.4.17.1 | hippuryl-DL-beta-phenylacetic acid + H2O | - |
Squalus acanthias | hippuric acid + DL-phenylacetic acid | - |
? | |
| 3.4.17.1 | hippuryl-DL-beta-phenyllactic acid + H2O | - |
Squalus acanthias | hippuric acid + 3-phenyllactic acid | - |
ir | |
| 3.4.17.1 | hippuryl-DL-phenyllactic acid + H2O | - |
Squalus acanthias | hippuric acid + DL-phenyllactic acid | - |
ir | |
| 3.4.17.1 | N-acetyl-Gly-L-phenyllactic acid + H2O | - |
Squalus acanthias | N-acetyl-Gly + L-phenyllactic acid | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.1 | monomer | SDS-PAGE | Squalus acanthias |
| 3.4.17.1 | More | tendency towards aggregation | Squalus acanthias |
| 3.4.17.1 | More | amino acid composition described | Squalus acanthias |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 25 | - |
assay at | Squalus acanthias |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.1 | 14.7 | - |
carbobenzoxy-Gly-L-Phe | - |
Squalus acanthias | |
| 3.4.17.1 | 86 | - |
N-acetyl-Gly-L-phenyllactic acid | - |
Squalus acanthias | |
| 3.4.17.1 | 635 | - |
hippuryl-DL-phenyllactic acid | - |
Squalus acanthias | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 7.5 | - |
assay at | Squalus acanthias |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.1 | 6.1 | - |
3-Phenylpropionic acid | - |
Squalus acanthias | |
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