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Literature summary extracted from

  • Everitt, M.T.; Neurath, H.
    Rat peritoneal mast cell carboxypeptidase: localization, purification, and enzymatic properties (1980), FEBS Lett., 110, 292-296.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.1 1,10-phenanthroline
-
 Rattus norvegicus
3.4.17.1 potato carboxypeptidase inhibitor
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.1 additional information
-
 additional information
-
 Rattus norvegicus
3.4.17.1 0.062
-
 hippuryl-L-phenyllactate
-
 Rattus norvegicus
3.4.17.1 0.14
-
 Benzyloxycarbonyl-Ala-Phe
-
 Rattus norvegicus
3.4.17.1 0.23
-
 Benzyloxycarbonyl-Phe-Leu
-
 Rattus norvegicus
3.4.17.1 0.31
-
 Benzyloxycarbonyl-Gly-Phe
-
 Rattus norvegicus
3.4.17.1 0.45
-
 benzoyl-Gly-L-Phe
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.1 secretory granule
-
 Rattus norvegicus 30141
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.1 35000
-
 in presence of urea and 2-mercaptoethanol, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.1 mast cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.1 7.54
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.1 benzoyl-Gly-L-Phe + H2O
-
 Rattus norvegicus benzoyl-Gly + L-Phe
-
 ir
3.4.17.1 Benzyloxycarbonyl-Ala-Phe + H2O
-
 Rattus norvegicus Benzyloxycarbonyl-Ala + Phe
-
 ir
3.4.17.1 Benzyloxycarbonyl-Gly-Phe + H2O
-
 Rattus norvegicus Benzyloxycarbonyl-Gly + Phe
-
 ir
3.4.17.1 benzyloxycarbonyl-Phe-Leu + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Phe + Leu
-
 ir
3.4.17.1 hippuryl-DL-phenyllactate + H2O
-
 Rattus norvegicus hippuric acid + DL-phenyllactate
-
 ir
3.4.17.1 hippuryl-L-phenyllactate + H2O
-
 Rattus norvegicus hippuric acid + L-phenyllactate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.1 More amino acid composition described Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.1 25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.1 4.2
-
 Benzyloxycarbonyl-Gly-Phe
-
 Rattus norvegicus
3.4.17.1 16
-
 benzoyl-Gly-L-Phe
-
 Rattus norvegicus
3.4.17.1 66
-
 Benzyloxycarbonyl-Phe-Leu
-
 Rattus norvegicus
3.4.17.1 89
-
 Benzyloxycarbonyl-Ala-Phe
-
 Rattus norvegicus
3.4.17.1 454
-
 hippuryl-L-phenyllactate
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.1 7 8.5 esterase activity, broad optimum Rattus norvegicus
3.4.17.1 8
-
 peptidase activity, sharp optimum Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.1 5.5 10 pH 5.5: about 35% of maximum activity, pH 10: about 40% of maximum activity Rattus norvegicus
3.4.17.1 6 9.5 pH 6.0, pH 9.5: about 25% of maximum peptidase activity Rattus norvegicus