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Literature summary extracted from

  • Odya, C.E.; Erdös, E.G.
    Human prolylcarboxypeptidase (1981), Methods Enzymol., 80, 460-466.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.2 diisopropyl fluorophosphate 1 mM, complete inhibition Homo sapiens
3.4.16.2 pepstatin 0.3 mM, complete inhibition Homo sapiens
3.4.16.2 phenylmethylsulfonyl fluoride 1 mM, complete inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.2 0.77
-
 N-benzyloxycarbonyl-Pro-Phe
-
 Homo sapiens
3.4.16.2 1
-
 angiotensin II
-
 Homo sapiens
3.4.16.2 2
-
 angiotensin III
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.16.2 45000
-
 x * 45000 + x * 66500, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
3.4.16.2 66500
-
 x * 45000 + x * 66500, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
3.4.16.2 115000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.16.2 blood
-
 Homo sapiens
-
3.4.16.2 fibroblast
-
 Homo sapiens
-
3.4.16.2 kidney
-
 Homo sapiens
-
3.4.16.2 lung
-
 Homo sapiens
-
3.4.16.2 lymphocyte
-
 Homo sapiens
-
3.4.16.2 macrophage
-
 Homo sapiens
-
3.4.16.2 polymorphonuclear leukocyte
-
 Homo sapiens
-
3.4.16.2 urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.16.2 0.51
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.2 angiotensin II + H2O about 35% of the activity with angiotensin III Homo sapiens ?
-
 ?
3.4.16.2 angiotensin III + H2O
-
 Homo sapiens ?
-
 ?
3.4.16.2 N-benzyloxycarbonyl-Pro-Ala + H2O 33% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Ala
-
 ?
3.4.16.2 N-benzyloxycarbonyl-Pro-Leu + H2O 12% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Leu
-
 ?
3.4.16.2 N-benzyloxycarbonyl-Pro-Phe + H2O 8% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Phe
-
 ?
3.4.16.2 N-benzyloxycarbonyl-Pro-Ser + H2O 7% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Ser
-
 ?
3.4.16.2 N-benzyloxycarbonyl-Pro-Val + H2O 28% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Val
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.16.2 ? x * 45000 + x * 66500, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.16.2 60
-
 30 min, 20% loss of activity Homo sapiens
3.4.16.2 70
-
 15 min, 90% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.2 4.5 5.5 with N-benzyloxycarbonyl-Pro-Phe, angiotensin II or angiotensin III as substrate Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.16.2 4.5 7
-
 Homo sapiens