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Literature summary extracted from

  • Zevaco, C.; Monnet, V.; Gripon, J.C.
    Intracellular X-prolyl dipeptidyl peptidase from Lactococcus lactis spp. lactis: purification and properties (1990), J. Appl. Bacteriol., 68, 357-366.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.14.11 DMSO up to 25%, enhances activity Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 diisopropyl fluorophosphate
-
 Lactococcus lactis
3.4.14.11 diisopropylfluorophosphate
-
 Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.5 intracellular
-
 Lactococcus lactis 5622
-
3.4.14.11 intracellular
-
 Lactococcus lactis 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 85000
-
 2 * 85000, SDS-PAGE Lactococcus lactis
3.4.14.5 190000
-
 gel filtration Lactococcus lactis
3.4.14.11 85000
-
 2 * 85000, SDS-PAGE with or without 2-mercaptoethanol Lactococcus lactis
3.4.14.11 190000
-
 gel filtration Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Lactococcus lactis
-
 spp. lactis NCDO 763
-
3.4.14.11 Lactococcus lactis
-
 ssp. lactis
-
3.4.14.11 Lactococcus lactis
-
 NCDO 763
-
3.4.14.11 Lactococcus lactis NCDO 763
-
 NCDO 763
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
 Lactococcus lactis
3.4.14.11
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 additional information
-
-
 Lactococcus lactis
3.4.14.11 additional information
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis NCDO 763 Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis NCDO 763 Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.5 beta-casomorphin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.14.5 beta-casomorphin + H2O
-
 Lactococcus lactis NCDO 763 ?
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis NCDO 763 Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.11 Arg-Pro 4-nitroanilide + H2O
-
 Lactococcus lactis Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.11 beta-casomorphin + H2O Tyr-Pro-Phe-Pro-Gly-Pro-Ile Lactococcus lactis ? the hydrolysis of the tripeptide Gly-Pro-Ile is slower ?
3.4.14.11 beta-casomorphin + H2O Tyr-Pro-Phe-Pro-Gly-Pro-Ile Lactococcus lactis ? after the release of the dipeptide Tyr-Pro, the resulting pentapeptide does not accumulate in the medium, rather the dipeptide Phe-Pro is released very rapidly ?
3.4.14.11 beta-casomorphin + H2O Tyr-Pro-Phe-Pro-Gly-Pro-Ile Lactococcus lactis NCDO 763 ? the hydrolysis of the tripeptide Gly-Pro-Ile is slower ?
3.4.14.11 Gly-Pro 4-nitroanilide + H2O
-
 Lactococcus lactis Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.11 L-Ala-L-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer 2 * 85000, SDS-PAGE Lactococcus lactis
3.4.14.11 dimer 2 * 85000, SDS-PAGE with or without 2-mercaptoethanol Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 40 45
-
 Lactococcus lactis
3.4.14.11 40
-
-
 Lactococcus lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.14.11 30 50 50% of maximal activity at 30°C, maximum at 40-50°C Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 8.5
-
-
 Lactococcus lactis
3.4.14.11 8.5
-
-
 Lactococcus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: optimum Lactococcus lactis
3.4.14.11 5.5 8.5 pH 5.5: 50% of maximal activity, pH 8.5: activity maximum Lactococcus lactis