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Literature summary extracted from
- Catalytic properties of X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis subsp. cremoris nTR (1992), Biosci. Biotechnol. Biochem., 56, 704-707.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.5 | Ala-Ala | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis |  |
| 3.4.14.5 | Ala-Pro | hydrolysis of with Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Cu2+ | - |
Lactococcus lactis | |
| 3.4.14.5 | Gly-Ala | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Gly-L-Pro | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Hg2+ | - |
Lactococcus lactis | |
| 3.4.14.5 | Leu-Ala | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Leu-Pro | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Phe-Pro | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Pro-Ala | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Pro-Gly | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Pro-Ile | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Pro-Leu | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Pro-Met | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Pro-Phe | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.5 | Tyr-Pro | hydrolysis of Gly-Pro-4-nitroanilide | Lactococcus lactis | |
| 3.4.14.11 | Cu2+ | - |
Lactococcus lactis | |
| 3.4.14.11 | Hg2+ | - |
Lactococcus lactis | |
| 3.4.14.11 | Urea | 2.0 M | Lactococcus lactis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.5 | additional information | - |
additional information | - |
Lactococcus lactis | |
| 3.4.14.11 | additional information | - |
additional information | - |
Lactococcus lactis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.14.5 | membrane | loosely bound to inner cell membrane | Lactococcus lactis | 16020 | - |
| 3.4.14.11 | inner membrane | loosely bound | Lactococcus lactis | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.5 | Lactococcus lactis | - |
subsp. cremonis nTR | - |
| 3.4.14.11 | Lactococcus lactis | - |
subsp. cremoris | - |
| 3.4.14.11 | Lactococcus lactis | - |
nTR | - |
| 3.4.14.11 | Lactococcus lactis nTR | - |
nTR | - |
| 3.4.14.11 | Lactococcus lactis subsp. cremoris | - |
subsp. cremoris | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.11 | - |
Lactococcus lactis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.14.11 | -20°C, 60% glycerol, stable for 30 days | Lactococcus lactis |
| 3.4.14.11 | 4°C, pH 8.0, stable | Lactococcus lactis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.5 | Ala-Ala-4-nitroanilide + H2O | - |
Lactococcus lactis | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Ala-Ala-4-nitroanilide + H2O | - |
Lactococcus lactis nTR | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Ala-Ala-4-nitroanilide + H2O | - |
Lactococcus lactis subsp. cremoris | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis nTR | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis subsp. cremoris | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | beta-casomorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| 3.4.14.5 | beta-casomorphin + H2O | - |
Lactococcus lactis nTR | ? | - |
? | |
| 3.4.14.5 | beta-casomorphin + H2O | - |
Lactococcus lactis subsp. cremoris | ? | - |
? | |
| 3.4.14.5 | Gly-L-Pro-L-Ala + H2O | - |
Lactococcus lactis | Gly-L-Pro + L-Ala | - |
? | |
| 3.4.14.5 | Gly-L-Pro-L-Ala + H2O | - |
Lactococcus lactis nTR | Gly-L-Pro + L-Ala | - |
? | |
| 3.4.14.5 | Gly-L-Pro-L-Ala + H2O | - |
Lactococcus lactis subsp. cremoris | Gly-L-Pro + L-Ala | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis nTR | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis subsp. cremoris | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | His-Pro-Leu + H2O | - |
Lactococcus lactis | ? | - |
? | |
| 3.4.14.5 | His-Pro-Tyr + H2O | - |
Lactococcus lactis | ? | - |
? | |
| 3.4.14.5 | Leu-Ala-Pro + H2O | - |
Lactococcus lactis | ? | - |
? | |
| 3.4.14.11 | Ala-Ala 4-nitroanilide + H2O | - |
Lactococcus lactis | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Arg-Pro 4-nitroanilide + H2O | - |
Lactococcus lactis | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | beta-casomorphin + H2O | - |
Lactococcus lactis | ? | the hydrolysis of the tripeptide Gly-Pro-Ile is slower | ? | |
| 3.4.14.11 | beta-casomorphin + H2O | - |
Lactococcus lactis | ? | after the release of the dipeptide Tyr-Pro, the resulting pentapeptide does not accumulate in the medium, rather the dipeptide Phe-Pro is released very rapidly | ? | |
| 3.4.14.11 | Gly-Pro 4-nitroanilide + H2O | - |
Lactococcus lactis | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Gly-Pro-Ala + H2O | - |
Lactococcus lactis | Gly-Pro + Ala | - |
? | |
| 3.4.14.11 | His-Pro-Leu + H2O | - |
Lactococcus lactis | His-Pro + Leu | - |
? | |
| 3.4.14.11 | His-Pro-Tyr + H2O | - |
Lactococcus lactis | His-Pro + Tyr | - |
? | |
| 3.4.14.11 | L-Ala-L-Ala-7-amido-4-methylcoumarin + H2O | - |
Lactococcus lactis | L-Ala-L-Ala + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.14.11 | Leu-Ala-Pro + H2O | - |
Lactococcus lactis | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.5 | 26 | - |
Ala-Ala-4-nitroanilide | - |
Lactococcus lactis | |
| 3.4.14.5 | 120 | - |
Leu-Ala-Pro | - |
Lactococcus lactis | |
| 3.4.14.5 | 178 | - |
Gly-Pro-4-nitroanilide | - |
Lactococcus lactis | |
| 3.4.14.5 | 181 | - |
Arg-Pro-4-nitroanilide | - |
Lactococcus lactis | |
| 3.4.14.5 | 418 | - |
His-Pro-Leu | - |
Lactococcus lactis | |
| 3.4.14.5 | 670 | - |
His-Pro-Tyr | - |
Lactococcus lactis | |
| 3.4.14.5 | 2180 | - |
Gly-Pro-Ala | - |
Lactococcus lactis | |
| 3.4.14.11 | 26 | - |
L-Ala-L-Ala-7-amido-4-methylcoumarin | - |
Lactococcus lactis | |
| 3.4.14.11 | 120 | - |
Leu-Ala-Pro | - |
Lactococcus lactis | |
| 3.4.14.11 | 178 | - |
Gly-Pro 4-nitroanilide | - |
Lactococcus lactis | |
| 3.4.14.11 | 181 | - |
Arg-Pro 4-nitroanilide | - |
Lactococcus lactis | |
| 3.4.14.11 | 418 | - |
His-Pro-Leu | - |
Lactococcus lactis | |
| 3.4.14.11 | 670 | - |
His-Pro-Tyr | - |
Lactococcus lactis | |
| 3.4.14.11 | 2180 | - |
Gly-Pro-Ala | - |
Lactococcus lactis | |
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