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Literature summary extracted from
- Purification and properties of dipeptidyl peptidase IV from Streptococcus mitis ATCC 9811 (1981), Arch. Biochem. Biophys., 210, 230-237.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.14.5 | treatment with trypsin, chymotrypsin A, pronase-P and papain completely destroy the activity | Streptococcus mitis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.5 | 1,10-phenanthroline | - |
Streptococcus mitis |  |
| 3.4.14.5 | Cu2+ | - |
Streptococcus mitis | |
| 3.4.14.5 | diisopropyl fluorophosphate | - |
Streptococcus mitis | |
| 3.4.14.5 | EDTA | - |
Streptococcus mitis | |
| 3.4.14.5 | Hg2+ | - |
Streptococcus mitis | |
| 3.4.14.5 | Pb2+ | - |
Streptococcus mitis | |
| 3.4.14.5 | Zn2+ | - |
Streptococcus mitis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.5 | 0.06 | - |
Gly-Pro-4-nitroanilide | - |
Streptococcus mitis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.5 | Fe2+ | slight increase in activity | Streptococcus mitis | |
| 3.4.14.5 | Mn2+ | slight increase in activity | Streptococcus mitis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 53000 | - |
x * 53000, SDS-PAGE | Streptococcus mitis |
| 3.4.14.5 | 120000 | - |
gel filtration | Streptococcus mitis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.5 | Streptococcus mitis | - |
ATCC 9811 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | no modification | no carbohydrate | Streptococcus mitis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.5 | - |
Streptococcus mitis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 56.2 | - |
- |
Streptococcus mitis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.5 | Ala-Ala-4-nitroanilide + H2O | 20% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Glu-Pro-4-nitroanilide + H2O | 23% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Glu-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-hydroxyproline-4-nitroanilide + H2O | 10% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Gly-hydroxyproline + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus mitis | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-Pro-Leu-Gly-Pro + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | ? | - |
? | |
| 3.4.14.5 | Lys-Pro-4-nitroanilide + H2O | 65% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Lys-Pro + 4-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | ? | x * 53000, SDS-PAGE | Streptococcus mitis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 50 | - |
stable below | Streptococcus mitis |
| 3.4.14.5 | 55 | - |
10 min, activity is almost lost | Streptococcus mitis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 6 | 8.7 | - |
Streptococcus mitis |
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