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Literature summary extracted from

  • Kikuchi, M.; Fukuyama, K.; Epstein, W.L.
    Soluble dipeptidyl peptidase IV from terminal differentiated rat epidermal cells: purification and its activity on synthetic and natural peptides (1988), Arch. Biochem. Biophys., 266, 369-376.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 Cd2+ 1 mM Rattus norvegicus
3.4.14.5 Co2+ 1 mM Rattus norvegicus
3.4.14.5 diisopropyl fluorophosphate
-
 Rattus norvegicus
3.4.14.5 Diprotin A
-
 Rattus norvegicus
3.4.14.5 Hg2+ 1 mM Rattus norvegicus
3.4.14.5 Ni2+ 1 mM Rattus norvegicus
3.4.14.5 phenylmethylsulfonyl fluoride slight Rattus norvegicus
3.4.14.5 Zn2+ 1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 0.025
-
 Tyr-Pro-Phe-Val-Glu-Pro-Ile
-
 Rattus norvegicus
3.4.14.5 0.05
-
 Arg-Pro-Lys-Pro
-
 Rattus norvegicus
3.4.14.5 0.05
-
 Tyr-Pro-Phe-Val-Glu-Pro-Ile
-
 Rattus norvegicus
3.4.14.5 0.05
-
 Tyr-Pro-Phe-Pro-Gly
-
 Rattus norvegicus
3.4.14.5 0.05
-
 Tyr-Pro-Phe
-
 Rattus norvegicus
3.4.14.5 0.05
-
 Tyr-Pro-Phe-Pro
-
 Rattus norvegicus
3.4.14.5 0.09
-
 Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
 Rattus norvegicus
3.4.14.5 0.15
-
 Arg-Pro-Lys-Pro-Gln-Gln-Phe
-
 Rattus norvegicus
3.4.14.5 0.18
-
 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly
-
 Rattus norvegicus
3.4.14.5 0.3
-
 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 90000
-
 2 * 90000, SDS-PAGE Rattus norvegicus
3.4.14.5 190000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.5 side-chain modification glycoprotein Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 epidermis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 31.9
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Arg-Pro-Lys-Pro + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Arg-Pro-Lys-Pro-Gln-Gln-Phe + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly
-
 Rattus norvegicus Arg-Pro + Lys-Pro-Gln-Gln-Phe-Phe-Gly
-
 ?
3.4.14.5 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
-
 Rattus norvegicus Arg-Pro + Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
 ?
3.4.14.5 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH3 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 beta-casomorphin + H2O beta-casomorphin from human, i.e. Tyr-Pro-Phe-Val-Glu-Pro-Ileu Rattus norvegicus ?
-
 ?
3.4.14.5 beta-casomorphin + H2O beta-casomorphin from bovine, i.e. Tyr-Pro-Phe-Val-Glu-Pro-Ile Rattus norvegicus ?
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 substance P + H2O
-
 Rattus norvegicus Arg-Pro + Lys-Pro + ?
-
 ?
3.4.14.5 Tyr-Pro-Phe + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Tyr-Pro-Phe-Pro + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Tyr-Pro-Phe-Pro-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Tyr-Pro-Phe-Val-Glu-Pro-Ile + H2O
-
 Rattus norvegicus Tyr-Pro + Phe-Val-Glu-Pro-Ile
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer 2 * 90000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 37
-
 pH 6.0-9.5, stable for 2 h Rattus norvegicus
3.4.14.5 45
-
 10 min, stable Rattus norvegicus
3.4.14.5 60
-
 10 min, 46% loss of activity Rattus norvegicus
3.4.14.5 70
-
 10 min, complete loss of activity Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.5 50.3
-
 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
 Rattus norvegicus
3.4.14.5 60.3
-
 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly
-
 Rattus norvegicus
3.4.14.5 62.3
-
 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Gly
-
 Rattus norvegicus
3.4.14.5 66.1
-
 Arg-Pro-Lys-Pro
-
 Rattus norvegicus
3.4.14.5 71.4
-
 Tyr-Pro-Phe-Val-Glu-Pro-Ile
-
 Rattus norvegicus
3.4.14.5 87.5
-
 Tyr-Pro-Phe
-
 Rattus norvegicus
3.4.14.5 88.3
-
 Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
 Rattus norvegicus
3.4.14.5 90.8
-
 Tyr-Pro-Phe-Pro
-
 Rattus norvegicus
3.4.14.5 102
-
 Tyr-Pro-Phe-Pro-Gly
-
 Rattus norvegicus
3.4.14.5 103
-
 Tyr-Pro-Phe-Val-Glu-Pro-Ileu
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7.5
-
 hydrolysis of Gly-Pro-4-nitroanilide, substance P or beta-casomorphin Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.5 6 9.5 at 37°C stable for 2 h, at 4°C stable for 3 days Rattus norvegicus