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Literature summary extracted from

  • Kiefer-Partsch, B.; Bockelmann, W.; Geis, A.; Teuber, M.
    Purification of an X-prolyl-dipeptidyl aminopeptidase from the cell wall proteolytic system of Lactococcus lactis subsp. Cremoris (1989), Appl. Microbiol. Biotechnol., 31, 75-78.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 diisopropyl fluorophosphate
-
 Lactococcus lactis
3.4.14.5 phenylmethylsulfonyl fluoride
-
 Lactococcus lactis
3.4.14.11 diisopropylfluorophosphate
-
 Lactococcus lactis
3.4.14.11 EDTA no inhibition Lactococcus lactis
3.4.14.11 phenylmethylsulfonyl fluoride
-
 Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.14.11 additional information metal-independent enzyme Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 90000
-
 2 * 90000, SDS-PAGE Lactococcus lactis
3.4.14.11 90000
-
 2 * 90000, SDS-PAGE Lactococcus lactis
3.4.14.11 160000 180000 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.14.5 additional information Lactococcus lactis possible role of the enzyme seems to match the high Pro content of beta-casein ?
-
 ?
3.4.14.5 additional information Lactococcus lactis P8-2-47 possible role of the enzyme seems to match the high Pro content of beta-casein ?
-
 ?
3.4.14.5 additional information Lactococcus lactis subsp. cremoris possible role of the enzyme seems to match the high Pro content of beta-casein ?
-
 ?
3.4.14.11 beta-casein + H2O Lactococcus lactis function of the enzyme is to cleave the proline-rich sequence of beta-casein ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Lactococcus lactis
-
 subsp. cremonis, P8-2-47
-
3.4.14.11 Lactococcus lactis
-
 subsp. cremoris
-
3.4.14.11 Lactococcus lactis
-
 P8-2-47
-
3.4.14.11 Lactococcus lactis P8-2-47
-
 P8-2-47
-
3.4.14.11 Lactococcus lactis subsp. cremoris
-
 subsp. cremoris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
 Lactococcus lactis
3.4.14.11
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 90
-
-
 Lactococcus lactis
3.4.14.11 90
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis P8-2-47 Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis subsp. cremoris Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 beta-casein + H2O cleavage of the proline-rich sequence Lactococcus lactis ?
-
 ?
3.4.14.5 beta-casein + H2O cleavage of the proline-rich sequence Lactococcus lactis P8-2-47 ?
-
 ?
3.4.14.5 beta-casein + H2O cleavage of the proline-rich sequence Lactococcus lactis subsp. cremoris ?
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis P8-2-47 Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis subsp. cremoris Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 additional information possible role of the enzyme seems to match the high Pro content of beta-casein Lactococcus lactis ?
-
 ?
3.4.14.5 additional information possible role of the enzyme seems to match the high Pro content of beta-casein Lactococcus lactis P8-2-47 ?
-
 ?
3.4.14.5 additional information possible role of the enzyme seems to match the high Pro content of beta-casein Lactococcus lactis subsp. cremoris ?
-
 ?
3.4.14.11 beta-casein + H2O function of the enzyme is to cleave the proline-rich sequence of beta-casein Lactococcus lactis ?
-
 ?
3.4.14.11 beta-casomorphin + H2O
-
 Lactococcus lactis ? main split products: Tyr-Pro and Phe-Pro ?
3.4.14.11 beta-casomorphin + H2O
-
 Lactococcus lactis P8-2-47 ? main split products: Tyr-Pro and Phe-Pro ?
3.4.14.11 beta-casomorphin + H2O
-
 Lactococcus lactis subsp. cremoris ? main split products: Tyr-Pro and Phe-Pro ?
3.4.14.11 L-Ala-L-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer 2 * 90000, SDS-PAGE Lactococcus lactis
3.4.14.11 dimer 2 * 90000, SDS-PAGE Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 45 50
-
 Lactococcus lactis
3.4.14.11 45
-
-
 Lactococcus lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 50
-
 rapid inactivation above Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7
-
-
 Lactococcus lactis
3.4.14.11 6.5 8
-
 Lactococcus lactis