EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.14.5 | EDTA | - |
Streptococcus thermophilus | |
3.4.14.5 | iodoacetate | - |
Streptococcus thermophilus | |
3.4.14.5 | phenylmethylsulfonyl fluoride | - |
Streptococcus thermophilus | |
3.4.14.11 | EDTA | 5 mM, 40% inhibition | Streptococcus thermophilus | |
3.4.14.11 | iodoacetate | 5 mM, 64% inhibition | Streptococcus thermophilus | |
3.4.14.11 | phenylmethylsulfonyl fluoride | 5 mM, complete inhibition | Streptococcus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.14.5 | 3.1 | - |
Gly-Pro-p-nitroanilide | - |
Streptococcus thermophilus | |
3.4.14.11 | 3.1 | - |
Gly-Pro-4-nitroanilide | pH 7.0, 50°C | Streptococcus thermophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.14.11 | intracellular | - |
Streptococcus thermophilus | 5622 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 80000 | - |
2 * 80000, SDS-PAGE | Streptococcus thermophilus |
3.4.14.5 | 160000 | - |
gel filtration | Streptococcus thermophilus |
3.4.14.11 | 80000 | - |
2 * 80000, SDS-PAGE | Streptococcus thermophilus |
3.4.14.11 | 160000 | - |
gel filtration | Streptococcus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.14.5 | Streptococcus thermophilus | - |
ACA-DC 4 | - |
3.4.14.5 | Streptococcus thermophilus ACA-DC 4 | - |
ACA-DC 4 | - |
3.4.14.11 | Streptococcus thermophilus | - |
ACA-DC 4 | - |
3.4.14.11 | Streptococcus thermophilus ACA-DC 4 | - |
ACA-DC 4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.14.5 | - |
Streptococcus thermophilus |
3.4.14.11 | - |
Streptococcus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 285 | - |
- |
Streptococcus thermophilus |
3.4.14.11 | 285 | - |
- |
Streptococcus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.14.5 | Ala-4-nitroanilide + H2O | 1% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ala + 4-nitroaniline | - |
? | |
3.4.14.5 | Ala-Pro-Gly + H2O | 72% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ala-Pro + Gly | - |
? | |
3.4.14.5 | Arg-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Arg + 4-nitroaniline | - |
? | |
3.4.14.5 | Arg-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | Arg + 4-nitroaniline | - |
? | |
3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | 55% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Arg-Pro + 4-nitroaniline | - |
? | |
3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | 55% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | Arg-Pro + 4-nitroaniline | - |
? | |
3.4.14.5 | Gly-Phe-4-nitroanilide + H2O | 1% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Gly-Phe + 4-nitroaniline | - |
? | |
3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus thermophilus | Gly-Pro + 4-nitroaniline | - |
? | |
3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus thermophilus ACA-DC 4 | Gly-Pro + 4-nitroaniline | - |
? | |
3.4.14.5 | Gly-Pro-Arg-Pro + H2O | as active as with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | ? | - |
? | |
3.4.14.5 | Gly-Pro-Arg-Pro + H2O | as active as with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | ? | - |
? | |
3.4.14.5 | Ile-Pro-Ile + H2O | 34% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | ? | - |
? | |
3.4.14.5 | Phe-4-nitroanilide + H2O | 7% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Phe + 4-nitroaniline | - |
? | |
3.4.14.5 | Phe-4-nitroanilide + H2O | 7% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | Phe + 4-nitroaniline | - |
? | |
3.4.14.5 | Pro-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Pro + 4-nitroaniline | - |
? | |
3.4.14.5 | Val-Pro-Leu + H2O | 83% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | ? | - |
? | |
3.4.14.11 | Ala-Pro-Gly + H2O | 72% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ala-Pro + Gly | - |
? | |
3.4.14.11 | Arg-Pro 4-nitroanilide + H2O | 55% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Arg-Pro + 4-nitroaniline | - |
? | |
3.4.14.11 | Gly-Pro 4-nitroanilide + H2O | - |
Streptococcus thermophilus | Gly-Pro + 4-nitroaniline | - |
? | |
3.4.14.11 | Gly-Pro-Arg-Pro + H2O | as active as Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Gly-Pro + Arg-Pro | - |
? | |
3.4.14.11 | Ile-Pro-Ile + H2O | 34% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ile-Pro + Ile | - |
? | |
3.4.14.11 | Val-Pro-Leu + H2O | 83% of the activity with Gly-Pro-nitroanilide | Streptococcus thermophilus | Val-Pro + Leu | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.14.5 | dimer | 2 * 80000, SDS-PAGE | Streptococcus thermophilus |
3.4.14.11 | dimer | 2 * 80000, SDS-PAGE | Streptococcus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 50 | - |
- |
Streptococcus thermophilus |
3.4.14.11 | 50 | - |
hydrolysis of Gly-Pro-4-nitroanilide | Streptococcus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 10 | 60 | 10°C: 17% of maximal activity, about about 40% of maximal activity at 25°C and at 60°C | Streptococcus thermophilus |
3.4.14.11 | 40 | 60 | 40°C: about 15% of maximal activity, 60°C: about 40% of maximal activity | Streptococcus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 50 | - |
10 min, stable | Streptococcus thermophilus |
3.4.14.5 | 60 | - |
1 min, in absence of substrate, 96% loss of activity | Streptococcus thermophilus |
3.4.14.5 | 70 | - |
1 min, complete loss of activity | Streptococcus thermophilus |
3.4.14.11 | 60 | - |
1 min in absence of substrate, 96% loss of activity | Streptococcus thermophilus |
3.4.14.11 | 70 | - |
1 min, complete loss of activity | Streptococcus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 7 | - |
- |
Streptococcus thermophilus |
3.4.14.11 | 7 | - |
hydrolysis of Gly-Pro-4-nitroanilide | Streptococcus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 5 | 9 | pH 5.0: about 15% of maximal activity, pH 9.0: about 85% of maximal activity | Streptococcus thermophilus |
3.4.14.11 | 6 | 9 | pH 6.0: about 90% of maximal activity, pH 9.0: about 85% of maximal activity, hydrolysis of Gly-Pro-4-nitroanilide | Streptococcus thermophilus |