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Literature summary extracted from
- Purification and characterization of enkephalinase B from rat brain membrane (1987), Biochim. Biophys. Acta, 925, 27-35.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.4 | 1,10-phenanthroline | - |
Rattus norvegicus |  |
| 3.4.14.4 | EDTA | - |
Rattus norvegicus | |
| 3.4.14.4 | p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
| 3.4.14.4 | Propioxanthin A and B | - |
Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.4 | 0.053 | - |
Met-enkephalin | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.14.4 | membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.4 | Co2+ | increase of activity | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.4 | 80000 | 86000 | gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.4 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.4 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.14.4 | brain | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.4 | angiotensin II + H2O | - |
Rattus norvegicus | Asp-Arg + Val-Tyr + Ile-His + Pro-Phe | - |
? | |
| 3.4.14.4 | Gly-Gly-Phe-Leu + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.14.4 | Met-enkephalin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.14.4 | additional information | hydrolysis depending on type of amino acid and chain length | Rattus norvegicus | ? | - |
? | |
| 3.4.14.4 | Trp-Met-Asp-Phe-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.14.4 | Tyr-Gly-Gly-Phe + H2O | - |
Rattus norvegicus | Tyr-Gly + Gly-Phe | - |
? | |
| 3.4.14.4 | Tyr-Gly-Gly-Phe-Leu + H2O | - |
Rattus norvegicus | Tyr-Gly + Gly-Phe-Leu | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.4 | monomer | 1 * ?, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.4 | 4 | 40 | - |
Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.4 | additional information | - |
additional information | - |
Rattus norvegicus | |
| 3.4.14.4 | 13.2 | - |
Tyr-Gly-Gly-Phe-Leu | - |
Rattus norvegicus | |
| 3.4.14.4 | 50 | - |
Gly-Gly-Phe-Leu | - |
Rattus norvegicus | |
| 3.4.14.4 | 167 | - |
Trp-Met-Asp-Phe-NH2 | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.4 | 6 | 6.5 | substrate Met-enkephalin | Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.4 | 6.5 | 8 | - |
Rattus norvegicus |
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