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Literature summary extracted from

  • Johnson, G.L.; Brown, J.L.
    Partial purification and characterization of two peptidases from Neurospora crassa (1974), Biochim. Biophys. Acta, 370, 530-540.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.13.9 instable during purification Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.12 EDTA
-
 Neurospora crassa
3.4.13.12 p-chloromercuribenzoate 100% inhibition at 1 mM Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.9 0.41
-
 L-Val-L-Pro
-
 Neurospora crassa
3.4.13.9 0.81
-
 Met-Pro
-
 Neurospora crassa
3.4.13.9 1.3
-
 Gly-L-Pro
-
 Neurospora crassa
3.4.13.12 0.25 0.31 Met-Ala
-
 Neurospora crassa
3.4.13.12 0.42 1.02 Met-Ile
-
 Neurospora crassa
3.4.13.12 0.57 0.6 Met-Ser
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.12 Co2+ 52% of activity with manganese Neurospora crassa
3.4.13.12 Fe2+ 21% of activity with manganese Neurospora crassa
3.4.13.12 Mn2+
-
 Neurospora crassa
3.4.13.12 Zn2+ 20% of activity with manganese Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.12 110000 114000 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.12 Met-amino acid + H2O Neurospora crassa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Neurospora crassa
-
-
-
3.4.13.12 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.9 partial Neurospora crassa
3.4.13.12 partial Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.9 217
-
-
 Neurospora crassa
3.4.13.12 200
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 Gly-L-Pro + H2O
-
 Neurospora crassa Gly + L-Pro
-
 ?
3.4.13.9 L-Met-Pro + H2O
-
 Neurospora crassa L-Met + Pro
-
 ?
3.4.13.9 L-Val-L-Pro + H2O
-
 Neurospora crassa L-Val + L-Pro
-
 ?
3.4.13.12 Ala-Ala + H2O
-
 Neurospora crassa Ala + Ala
-
 ir
3.4.13.12 Ala-Leu + H2O
-
 Neurospora crassa Ala + Leu
-
 ir
3.4.13.12 Ala-Met + H2O
-
 Neurospora crassa Ala + Met
-
 ir
3.4.13.12 ethionyl-Ala + H2O
-
 Neurospora crassa ethionine + Ala
-
 ir
3.4.13.12 Leu-Ala + H2O
-
 Neurospora crassa Leu + Ala
-
 ir
3.4.13.12 Met-Ala + H2O best substrate Neurospora crassa Met + Ala
-
 ir
3.4.13.12 Met-amino acid + H2O
-
 Neurospora crassa ?
-
 ?
3.4.13.12 Met-Ile + H2O
-
 Neurospora crassa Met + Ile
-
 ir
3.4.13.12 Met-Leu + H2O
-
 Neurospora crassa Met + Leu
-
 ir
3.4.13.12 Met-Met + H2O
-
 Neurospora crassa Met + Met
-
 ir
3.4.13.12 Met-Ser + H2O
-
 Neurospora crassa Met + Ser
-
 ir
3.4.13.12 Met-Thr + H2O
-
 Neurospora crassa Met + Thr
-
 ir
3.4.13.12 norleucyl-Ala + H2O
-
 Neurospora crassa norleucine + Ala
-
 ir
3.4.13.12 Ser-Ala + H2O
-
 Neurospora crassa Ser + Ala
-
 ir
3.4.13.12 Thr-Ala + H2O
-
 Neurospora crassa Thr + Ala
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.12 8.5
-
 substrate Met-Ala Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.13.12 5.5 9.5 substrate Met-Ala Neurospora crassa