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Literature summary extracted from

  • Yoshimoto, T.; Saeki, T.; Tsuru, D.
    Proline iminopeptidase from Bacillus megaterium: purification and characterization (1983), J. Biochem., 93, 469-477.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.5 2-mercaptoethanol restores activity after deactivation with p-chloromercuribenzoate Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 Cu2+
-
 Priestia megaterium
3.4.11.5 Hg2+
-
 Priestia megaterium
3.4.11.5 p-chloromercuribenzoate
-
 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.5 0.152
-
 L-Pro-2-naphthylamide
-
 Priestia megaterium
3.4.11.5 0.32
-
 L-Pro-4-nitroanilide
-
 Priestia megaterium
3.4.11.5 0.78
-
 Pro-D-Ala
-
 Priestia megaterium
3.4.11.5 0.83
-
 Pro-Trp
-
 Priestia megaterium
3.4.11.5 0.9
-
 Pro-Asp
-
 Priestia megaterium
3.4.11.5 1.08
-
 Pro-Phe
-
 Priestia megaterium
3.4.11.5 1.15
-
 Pro-Gly
-
 Priestia megaterium
3.4.11.5 1.55
-
 Pro-Lys
-
 Priestia megaterium
3.4.11.5 1.86
-
 Pro-D-Phe
-
 Priestia megaterium
3.4.11.5 2.69
-
 Pro-Ala
-
 Priestia megaterium
3.4.11.5 3.6
-
 L-Pro-L-Leu-Gly-NH2
-
 Priestia megaterium
3.4.11.5 5.3
-
 Pro-Leu
-
 Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 58000
-
 gel filtration, SDS-PAGE Priestia megaterium
3.4.11.5 60000
-
 1 * 60000, SDS-PAGE Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Priestia megaterium
-
 var. polyliminolyticus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.5
-
 Priestia megaterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.4.11.5 5°C Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 L-Pro-2-naphthylamide + H2O
-
 Priestia megaterium L-Pro + 2-naphthylamine
-
 ?
3.4.11.5 L-Pro-4-nitroanilide + H2O
-
 Priestia megaterium L-Pro + 4-nitroaniline
-
 ?
3.4.11.5 L-Pro-L-Leu-Gly-NH2 + H2O
-
 Priestia megaterium L-Pro + L-Leu-Gly-NH2
-
 ?
3.4.11.5 L-prolyl-peptide + H2O cleavage of D-Pro residues Priestia megaterium L-proline + peptide
-
 ?
3.4.11.5 L-prolyl-peptide + H2O influence of amino acid adjacent to N-terminal L-Pro on rate of hydrolysis Priestia megaterium L-proline + peptide
-
 ?
3.4.11.5 N-L-Pro-2-naphthylamide + H2O
-
 Priestia megaterium L-proline + naphthylamine
-
 ?
3.4.11.5 Pro-Ala + H2O
-
 Priestia megaterium Pro + Ala
-
 ?
3.4.11.5 Pro-Asp + H2O
-
 Priestia megaterium Pro + Asp
-
 ?
3.4.11.5 Pro-D-Ala + H2O
-
 Priestia megaterium Pro + D-Ala
-
 ?
3.4.11.5 Pro-D-Phe + H2O
-
 Priestia megaterium Pro + D-Phe
-
 ?
3.4.11.5 Pro-Gly + H2O
-
 Priestia megaterium Pro + Gly
-
 ?
3.4.11.5 Pro-Leu + H2O
-
 Priestia megaterium Pro + Leu
-
 ?
3.4.11.5 Pro-Lys + H2O
-
 Priestia megaterium Pro + Lys
-
 ?
3.4.11.5 Pro-Phe + H2O
-
 Priestia megaterium Pro + Phe
-
 ?
3.4.11.5 Pro-Trp + H2O
-
 Priestia megaterium Pro + Trp
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.5 monomer 1 * 60000, SDS-PAGE Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.5 30
-
 pH 7, 30 min, 50% activity Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 7
-
-
 Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.5 6.5 7.5
-
 Priestia megaterium