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Literature summary extracted from

  • Waters, S.P.; Dalling, M.J.
    Purification and characterization of an iminopeptidase from the primary leaf of wheat (Triticum aestivum L.) (1983), Plant Physiol., 73, 1048-1054.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 Ag+
-
 Triticum aestivum
3.4.11.5 Bathocuproine inhibitor binds specifically to Cu2+ Triticum aestivum
3.4.11.5 Cu2+
-
 Triticum aestivum
3.4.11.5 N-ethylmaleimide
-
 Triticum aestivum
3.4.11.5 p-chloromercuribenzoate
-
 Triticum aestivum
3.4.11.5 Pb2+
-
 Triticum aestivum
3.4.11.5 Zn2+
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.5 0.01
-
 L-Pro-2-naphthylamide
-
 Triticum aestivum
3.4.11.5 0.125
-
 Hyp-2-naphthylamide
-
 Triticum aestivum
3.4.11.5 1.79
-
 Pro-Gly
-
 Triticum aestivum
3.4.11.5 27.8
-
 Hyp-Gly
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 400000
-
 gradient gel electrophoresis Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.5
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.5 leaf of 7 days old seedlings Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.5 additional information
-
-
 Triticum aestivum
3.4.11.5 68.3
-
 Pro-p-nitroanilide Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
3.4.11.5 -15°C, 50% v/v glycerol Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 Hyp-2-naphthylamide + H2O
-
 Triticum aestivum Hyp + 2-naphthylamine
-
 ?
3.4.11.5 Hyp-Gly + H2O
-
 Triticum aestivum Hyp + Gly
-
 ?
3.4.11.5 L-hydroxyproline-2-naphthylamide + H2O
-
 Triticum aestivum L-hydroxyproline + 2-naphthylamine
-
 ?
3.4.11.5 L-Pro-2-naphthylamide + H2O
-
 Triticum aestivum L-Pro + 2-naphthylamine
-
 ?
3.4.11.5 L-prolyl-peptide + H2O
-
 Triticum aestivum L-proline + peptide
-
 ?
3.4.11.5 N-L-Pro-2-naphthylamide + H2O
-
 Triticum aestivum L-proline + naphthylamine
-
 ?
3.4.11.5 Pro-Gly + H2O
-
 Triticum aestivum Pro + Gly
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.5 40
-
 120 min Triticum aestivum
3.4.11.5 50
-
 120 min, 70% activity Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 7.4
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.5 5.1
-
 no activity below Triticum aestivum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.5 7.25 9
-
 Triticum aestivum