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Literature summary extracted from
- Thermophilic aminopeptidase from Bacillus stearothermophilus. IV (1974), Int. J. Pept. Protein Res., 6, 499-514.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.4 | 1,10-phenanthroline | - |
Geobacillus stearothermophilus |  |
| 3.4.11.4 | EDTA | - |
Geobacillus stearothermophilus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.4 | cytoplasm | - |
Geobacillus stearothermophilus | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.4 | 47500 | - |
2 * 47500, SDS-PAGE | Geobacillus stearothermophilus |
| 3.4.11.4 | 108000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.4 | Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.4 | - |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.4 | additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.4 | Gly-Leu-Tyr + H2O | - |
Geobacillus stearothermophilus | Gly + Leu-Tyr | - |
? | |
| 3.4.11.4 | Leu-Gly-Gly + H2O | D-Leu | Geobacillus stearothermophilus | Leu + Gly-Gly | - |
? | |
| 3.4.11.4 | additional information | - |
Geobacillus stearothermophilus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.4 | dimer | 2 * 47500, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.4 | 60 | 70 | inactivation above | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.4 | 7 | - |
substrate Gly-Leu-Tyr | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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