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Literature summary extracted from

  • Chapot-Chartier, M.P.; Rul, F.; Nardi, M.; Gripon, J.C.
    Gene cloning and characterization of PepC, a cysteine aminopeptidase from Streptococcus thermophilus, with sequence similarity to the eucaryotic bleomycin hydrolase (1994), J. Biochem., 224, 497-506.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.3 expression in Escherichia coli Streptococcus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.3 Hg2+
-
 Streptococcus thermophilus
3.4.11.3 iodoacetic acid
-
 Streptococcus thermophilus
3.4.11.3 L-trans-epoxysuccinyl-leucinamido(4-guanidino)-butane E64 Streptococcus thermophilus
3.4.11.3 N-alpha-tosyl-L-lysylchloromethane
-
 Streptococcus thermophilus
3.4.11.3 N-ethylmaleimide
-
 Streptococcus thermophilus
3.4.11.3 p-hydroxymercuribenzoate
-
 Streptococcus thermophilus
3.4.11.3 tosylphenylalanine-chloromethane
-
 Streptococcus thermophilus
3.4.11.3 Zn2+
-
 Streptococcus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.3 3
-
 L-Lysine-p-nitroanilide
-
 Streptococcus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.3 50000
-
 6 * 50000, SDS-PAGE Streptococcus thermophilus
3.4.11.3 300000
-
 gel filtration Streptococcus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.3 Streptococcus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.3 expressed in Escherichia coli Streptococcus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.3 additional information
-
-
 Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.3 L-lysine-p-nitroanilide + H2O
-
 Streptococcus thermophilus L-lysine + p-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.3 hexamer 6 * 50000, SDS-PAGE Streptococcus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.3 37
-
-
 Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.3 7
-
-
 Streptococcus thermophilus