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Literature summary extracted from

  • Yoshimoto, T.; Tamesa, Y.; Gushi, K.; Murayama, N.; Tsuru, D.
    An aminopeptidase N from Escherichia coli HB1010: Purification and demonstration that the enzyme possesses arylamidase and peptidase activities (1988), Agric. Biol. Chem., 52, 217-225.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline
-
 Escherichia coli
3.4.11.2 4-chloromercuribenzoate
-
 Escherichia coli
3.4.11.2 amastatin
-
 Escherichia coli
3.4.11.2 bestatin
-
 Escherichia coli
3.4.11.2 Cu2+
-
 Escherichia coli
3.4.11.2 Hg2+
-
 Escherichia coli
3.4.11.2 puromycin
-
 Escherichia coli
3.4.11.2 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.05
-
 Leu-2-naphthylamide
-
 Escherichia coli
3.4.11.2 0.74
-
 Gly-2-naphthylamide
-
 Escherichia coli
3.4.11.2 1
-
 Ala-2-naphthylamide
-
 Escherichia coli
3.4.11.2 2.86
-
 Ala-p-nitroanilide
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Co2+ reactivation after inhibition by 1,10-phenanthroline Escherichia coli
3.4.11.2 Zn2+ at concentrations between 0.1 and 0.3 mM reactivation after inhibition by 1,10-phenanthroline, inhibitory at higher concentrations Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 80000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 aminoacyl peptide Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.2 0.78
-
 Gly-2-naphthylamide as substrate Escherichia coli
3.4.11.2 1.6
-
 Met-2-naphthylamide as substrate Escherichia coli
3.4.11.2 1.68
-
 Ala-p-nitroanilide as substrate Escherichia coli
3.4.11.2 3.54
-
 Ala-2-naphthylamide as substrate Escherichia coli
3.4.11.2 4.8
-
 Leu-2-naphthylamide as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Ala-2-naphthylamide + H2O
-
 Escherichia coli Ala + 2-naphthylamine
-
 ?
3.4.11.2 Ala-p-nitroanilide + H2O
-
 Escherichia coli Ala + p-nitroaniline
-
 ?
3.4.11.2 aminoacyl peptide
-
 Escherichia coli ?
-
 ?
3.4.11.2 dipeptides + H2O not: Leu-Pro Escherichia coli amino acids
-
 ?
3.4.11.2 Gly-2-naphthylamide + H2O
-
 Escherichia coli Gly + 2-naphthylamine
-
 ?
3.4.11.2 Leu-2-naphthylamide + H2O
-
 Escherichia coli Leu + 2-naphthylamine
-
 ?
3.4.11.2 Leu-Gly + H2O
-
 Escherichia coli Leu + Gly
-
 ?
3.4.11.2 Met-2-naphthylamide + H2O
-
 Escherichia coli Met + 2-naphthylamine
-
 ?
3.4.11.2 tripeptides + H2O not: Gly-Pro-Ala Escherichia coli dipeptides + amino acids
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer 1 * 80000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 40
-
 activity was measured for 5 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7.5
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.2 7.5 9 stable for 1h at 30°C Escherichia coli