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Literature summary extracted from

  • Wachsmuth, E.D.; Fritze, I.; Pfleiderer, G.
    An aminopeptidase occurring in pig kidney. I. An improved method of preparation. Physical and enzymic properties (1966), Biochemistry, 5, 169-174.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.11.2 6 M urea, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.2 1 Leu-p-nitroanilide Km is pH-dependent between pH 6 and pH 9, maximum affinity at pH 7.0-7.3 Sus scrofa
3.4.11.2 0.6 6 Ala-p-nitroanilide Km is pH-dependent between pH 6 and pH 9, maximum affinity at pH 7.0-7.3 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 microsome
-
 Sus scrofa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 280000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 aminoacyl peptide Sus scrofa
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.11.2 Acetone 35 vol.%, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa
3.4.11.2 chloroform saturated solution in H2O, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa
3.4.11.2 toluene saturated solution in H2O, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Ala-p-nitroanilide + H2O
-
 Sus scrofa Ala + p-nitroaniline
-
 ?
3.4.11.2 aminoacyl peptide
-
 Sus scrofa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 60
-
 substrate Leu-p-nitroanilide Sus scrofa
3.4.11.2 70
-
 substrate Gly-p-nitroanilide Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.2 65
-
 stable Sus scrofa
3.4.11.2 70
-
 50% loss of activity after 10 min Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.2 174
-
 Leu-p-nitroanilide
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 8.5 9 maximum rate of hydrolysis of Leu-p-nitroanilide or Ala-p-nitroanilide, maximum substrate affinity at pH 7.0-7.3 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.2 3.5 11 stable for at least 3 h Sus scrofa