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Literature summary extracted from

  • McClellan, J.B.; Garner, C.W.
    Purification and properties of human intestine alanine aminopeptidase (1980), Biochim. Biophys. Acta, 613, 160-167.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline 99% inhibition at 0.4 mM, Ki: 0.067 mM Homo sapiens
3.4.11.2 2,2'-bipyridine 90% inhibition at 5 mM Homo sapiens
3.4.11.2 8-hydroxyquinoline 91% inhibition at 3 mM Homo sapiens
3.4.11.2 EDTA 14% inhibition at 1 mM Homo sapiens
3.4.11.2 Methicillin Ki: 4.61 mM Homo sapiens
3.4.11.2 phenylalanine Ki: 0.67 mM Homo sapiens
3.4.11.2 puromycin Ki: 0.0125 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.02
-
 L-Met-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.025
-
 Lys-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.067
-
 Leu-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.07
-
 L-Val-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.088
-
 L-Arg-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.091
-
 Ala-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.092
-
 L-Ile-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.108
-
 Ser-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.202
-
 Phe-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.367
-
 L-His-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.617
-
 L-Thr-2-naphthylamide
-
 Homo sapiens
3.4.11.2 0.647
-
 Leu-p-nitroanilide
-
 Homo sapiens
3.4.11.2 0.74
-
 Gly-4-nitroanilide
-
 Homo sapiens
3.4.11.2 0.785
-
 L-Asn-2-naphthylamide
-
 Homo sapiens
3.4.11.2 2.33
-
 L-Glu-2-naphthylamide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 membrane mucosal membrane Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Co2+ increase of activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 108000
-
 2 * 108000, SDS-PAGE Homo sapiens
3.4.11.2 206000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 aminoacyl peptide Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Ala-2-naphthylamide + H2O
-
 Homo sapiens Ala + 2-naphthylamine
-
 ?
3.4.11.2 aminoacyl peptide
-
 Homo sapiens ?
-
 ?
3.4.11.2 aminoacyl-2-naphthylamide + H2O preference for substrates having hydrophobic or basic amino terminal residues with no beta-branching Homo sapiens amino acid + 2-naphthylamine
-
 ?
3.4.11.2 aminoacyl-p-nitroanilide + H2O
-
 Homo sapiens amino acid + nitroaniline
-
 ?
3.4.11.2 Gly-4-nitroanilide + H2O
-
 Homo sapiens Gly + 4-nitroaniline
-
 ?
3.4.11.2 L-Arg-2-naphthylamide + H2O
-
 Homo sapiens L-Arg + 2-naphthylamine
-
 ?
3.4.11.2 L-Asn-2-naphthylamide + H2O
-
 Homo sapiens L-Asn + 2-naphthylamine
-
 ?
3.4.11.2 L-Glu-2-naphthylamide + H2O
-
 Homo sapiens L-Glu + 2-naphthylamine
-
 ?
3.4.11.2 L-His-2-naphthylamide + H2O
-
 Homo sapiens L-His + 2-naphthylamine
-
 ?
3.4.11.2 L-Ile-2-naphthylamide + H2O
-
 Homo sapiens L-Ile + 2-naphthylamine
-
 ?
3.4.11.2 L-Lys-2-naphthylamide + H2O
-
 Homo sapiens L-Lys + 2-naphthylamine
-
 ?
3.4.11.2 L-Met-2-naphthylamide + H2O
-
 Homo sapiens L-Met + 2-naphthylamine
-
 ?
3.4.11.2 L-Phe-2-naphthylamide + H2O
-
 Homo sapiens L-Phe + 2-naphthylamine
-
 ?
3.4.11.2 L-Thr-2-naphthylamide + H2O
-
 Homo sapiens L-Thr + 2-naphthylamine
-
 ?
3.4.11.2 L-Val-2-naphthylamide + H2O
-
 Homo sapiens L-Val + 2-naphthylamine
-
 ?
3.4.11.2 Leu-2-naphthylamide + H2O
-
 Homo sapiens Leu + 2-naphthylamine
-
 ?
3.4.11.2 Ser-beta-naphthylamide + H2O
-
 Homo sapiens Ser + beta-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 dimer 2 * 108000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.2 0.0125
-
 puromycin
-
 Homo sapiens
3.4.11.2 0.067
-
 1,10-phenanthroline
-
 Homo sapiens
3.4.11.2 0.67
-
 phenylalanine
-
 Homo sapiens
3.4.11.2 4.61
-
 Methicillin
-
 Homo sapiens